A solid-phase screening method for identification of glycan-binding cells
- Authors
- Kim, Yoon-Woo; Kim, Kyoung-Jin; Hwang, Cheol-Hwan; Kim, Yun-Gon
- Issue Date
- Apr-2015
- Publisher
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
- Keywords
- glycan-binding cells; N-glycan; pig kidney; alpha-galactose; immobilization
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.20, no.2, pp.366 - 372
- Journal Title
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
- Volume
- 20
- Number
- 2
- Start Page
- 366
- End Page
- 372
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/8774
- DOI
- 10.1007/s12257-014-0662-8
- ISSN
- 1226-8372
- Abstract
- Glycan immobilization and epitope recognition on a solid support is useful for functional glycoproteomics and analysis of protein-protein interactions. However, the lack of high-throughput experimental methods for the examination of cell-glycan interactions means that identifying glycan-binding cells remains a challenge. Herein, we describe a tool which enables the screening of glycanbinding cells that specifically recognize the glycan epitopes released from the membrane glycoproteins of cells and tissues. Biotinylated pig kidney N-glycans were immobilized on streptavidin-conjugated solid supports and then incubated with human immune cell lines. U937 cells discriminated between the alpha-galactose (alpha-Gal) and non-Gal pig N-glycans on the solid support. This screening strategy could aid exploration of cell-protein or cell-cell interactions.
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Collections - College of Engineering > Department of Chemical Engineering > 1. Journal Articles
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