Comparative N-Linked Glycan Analysis of Wild-Type and alpha 1,3-Galactosyltransferase Gene Knock-Out Pig Fibroblasts Using Mass Spectrometry Approaches
DC Field | Value | Language |
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dc.contributor.author | Park, Hae-Min | - |
dc.contributor.author | Kim, Yoon-Woo | - |
dc.contributor.author | Kim, Kyoung-Jin | - |
dc.contributor.author | Kim, Young June | - |
dc.contributor.author | Yang, Yung-Hun | - |
dc.contributor.author | Jin, Jang Mi | - |
dc.contributor.author | Kim, Young Hwan | - |
dc.contributor.author | Kim, Byung-Gee | - |
dc.contributor.author | Shim, Hosup | - |
dc.contributor.author | Kim, Yun-Gon | - |
dc.date.available | 2018-05-09T07:43:03Z | - |
dc.date.created | 2018-04-17 | - |
dc.date.issued | 2015-01 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/8839 | - |
dc.description.abstract | Carbohydrate antigens expressed on pig cells are considered to be major barriers in pig-to-human xenotransplantation. Even after alpha 1,3-galactosyltransferase gene knock-out (GalT-KO) pigs are generated, potential non-Gal antigens are still existed. However, to the best of our knowledge there is no extensive study analyzing N-glycans expressed on the GalT-KO pig tissues or cells. Here, we identified and quantified totally 47 N-glycans from wild-type (WT) and GalT-KO pig fibroblasts using mass spectrometry. First, our results confirmed the absence of galactose-alpha-1,3-galactose (alpha-Gal) residue in the GalT-KO pig cells. Interestingly, we showed that the level of overall fucosylated N-glycans from GalT-KO pig fibroblasts is much higher than from WT pig fibroblasts. Moreover, the relative quantity of the N-glycolylneuraminic acid (NeuGc) antigen is slightly higher in the GalT-KO pigs. Thus, this study will contribute to a better understanding of cellular glycan alterations on GalT-KO pigs for successful xenotransplantation. | - |
dc.publisher | KOREAN SOC MOLECULAR & CELLULAR BIOLOGY | - |
dc.relation.isPartOf | MOLECULES AND CELLS | - |
dc.subject | SOLID-PHASE PERMETHYLATION | - |
dc.subject | HUMAN NATURAL ANTIBODIES | - |
dc.subject | ALPHA-GAL | - |
dc.subject | NUCLEAR TRANSFER | - |
dc.subject | ENDOTHELIAL-CELLS | - |
dc.subject | DEFICIENT PIGS | - |
dc.subject | GLYCOSYLATION | - |
dc.subject | QUANTIFICATION | - |
dc.subject | KIDNEY | - |
dc.subject | XENOTRANSPLANTATION | - |
dc.title | Comparative N-Linked Glycan Analysis of Wild-Type and alpha 1,3-Galactosyltransferase Gene Knock-Out Pig Fibroblasts Using Mass Spectrometry Approaches | - |
dc.type | Article | - |
dc.identifier.doi | 10.14348/molcells.2015.2240 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | MOLECULES AND CELLS, v.38, no.1, pp.65 - 74 | - |
dc.identifier.kciid | ART001957838 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000349202900009 | - |
dc.identifier.scopusid | 2-s2.0-84948751760 | - |
dc.citation.endPage | 74 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 65 | - |
dc.citation.title | MOLECULES AND CELLS | - |
dc.citation.volume | 38 | - |
dc.contributor.affiliatedAuthor | Kim, Yun-Gon | - |
dc.type.docType | Article | - |
dc.description.oadoiVersion | published | - |
dc.subject.keywordAuthor | GalT-KO pig fibroblast | - |
dc.subject.keywordAuthor | mass spectrometry (MS) | - |
dc.subject.keywordAuthor | N-glycan | - |
dc.subject.keywordAuthor | N-glycolylneuraminic acid (NeuGc) | - |
dc.subject.keywordPlus | SOLID-PHASE PERMETHYLATION | - |
dc.subject.keywordPlus | HUMAN NATURAL ANTIBODIES | - |
dc.subject.keywordPlus | ALPHA-GAL | - |
dc.subject.keywordPlus | NUCLEAR TRANSFER | - |
dc.subject.keywordPlus | ENDOTHELIAL-CELLS | - |
dc.subject.keywordPlus | DEFICIENT PIGS | - |
dc.subject.keywordPlus | GLYCOSYLATION | - |
dc.subject.keywordPlus | QUANTIFICATION | - |
dc.subject.keywordPlus | KIDNEY | - |
dc.subject.keywordPlus | XENOTRANSPLANTATION | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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