MALDI-MS 기반 당단백질 당쇄의 정량분석 기술 개발 연구 동향Recent Advances in MALDI-MS Based Quantitative Targeted Glycan Analysis
- Other Titles
- Recent Advances in MALDI-MS Based Quantitative Targeted Glycan Analysis
- Authors
- 김경진; 김윤우; 황철환; 박한규; 정재현; 김윤곤
- Issue Date
- Oct-2015
- Publisher
- 한국생물공학회
- Keywords
- MALDI-MS; Recombinant glycoprotein drug; Glycan; Quantitative analysis; Chemical derivatization; Highthroughput analysis
- Citation
- KSBB Journal, v.30, no.5, pp.230 - 238
- Journal Title
- KSBB Journal
- Volume
- 30
- Number
- 5
- Start Page
- 230
- End Page
- 238
- URI
- http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/9556
- ISSN
- 1225-7117
- Abstract
- Abnormal glycosylation can significantly affect the intrinsic functions (i.e., stability and solubility) of proteins and the extrinsic protein interactions with other biomolecules. For example, recombinant glycoprotein therapeutics needs proper glycosylation for optimal drug efficacy. Therefore, there has been a strong demand for rapid, sensitive and high-throughput glycomics tools for real-time monitoring and fast validation of the biotherapeutics glycosylation. Although liquid chromatography tandem mass spectrometry (LC-MS/MS) is one of the most powerful tools for the characterization of glycan structures, it is generally time consuming and requires highly skilled personnel to collect the data and analyze the results. Recently, as an alternative method, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS), which is a fast, robust and easy-to-use instrumentation, has been used for quantitative glycomics with various chemical derivatization techniques. In this review, we highlight the recent advances in MALDI-MS based quantitative glycan analysis according to the chemical derivatization strategies. Moreover, we address the application of MALDIMS for high-throughput glycan analysis in many fields of clinical and biochemical engineering.
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