AP-1-Targeted Inhibition of Macrophage Function and Lipopolysaccharide/D-Galactosamine-Induced Hepatitis by Phyllanthus acidus Methanolic Extract
DC Field | Value | Language |
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dc.contributor.author | Hossen, Muhammad Jahangir | - |
dc.contributor.author | Kim, Mi-Yeon | - |
dc.contributor.author | Kim, Jong-Hoon | - |
dc.contributor.author | Cho, Jae Youl | - |
dc.date.available | 2018-05-09T08:54:04Z | - |
dc.date.created | 2018-04-17 | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 0192-415X | - |
dc.identifier.uri | http://scholarworks.bwise.kr/ssu/handle/2018.sw.ssu/9847 | - |
dc.description.abstract | Traditionally, Phyllanthus acidus (Phyllanthaceae) has been used for the treatment of rheumatism, bronchitis, asthma, respiratory disorders, and hepatitis. Recently, we showed that a methanol extract of Phyllanthaceae (Pa-ME) has a potent anti-inflammatory activity in RAW264.7 cells and strongly ameliorates HCl/EtOH-induced gastric ulcers in mice by targeting the Src/Syk of NF-kappa B. In the present study, we explored the molecular mechanism of Pa-ME on the AP-1 activation pathway and evaluated its potential hepatoprotective effects. To do this, we employed lipopolysaccharide (LPS)-stimulated RAW264.7 cells and U937 cells and an LPS/D-galactosamine (D-GaIN)-induced acute hepatitis mouse model. We utilized a multitude of assays, including immunoblotting analysis, reporter gene assays, and mRNA expression analysis, to determine the effect of Pa-ME on the AP-1 pathway. Pa-ME strikingly suppressed the production of LPS-induced pro-inflammatory cytokines including interleukin (IL)-1 beta, IL-6, and tumor necrosis factor-alpha (TNF-alpha). Furthermore, Pa-ME also strongly inhibited activator protein-1 (AP-1) activation and mitogen-activated protein kinase (MAPK) phosphorylation in LPS-stimulated RAW264.7 macrophages cells and the U937 monocyte like human cell line. Moreover, pre-treatment with Pa-ME exhibited strong hepatoprotective and curative effects in an LPS/D-Gal-induced mouse hepatitis model as evidenced by a decrease in elevated serum AST and ALT levels and the amelioration of histological damage. Taken together, our data suggest that Pa-ME might play a crucial ethnopharmacological role as a hepatoprotective herbal remedy by suppressing MAPK signaling and the activity of the downstream transcription factor AP-1. | - |
dc.publisher | WORLD SCIENTIFIC PUBL CO PTE LTD | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF CHINESE MEDICINE | - |
dc.subject | NF-KAPPA-B | - |
dc.subject | ETHANOL EXTRACT | - |
dc.subject | IN-VITRO | - |
dc.subject | ANTIINFLAMMATORY ACTIVITIES | - |
dc.subject | MOLECULAR-MECHANISMS | - |
dc.subject | SIGNALING PATHWAYS | - |
dc.subject | LIVER-INJURY | - |
dc.subject | ACTIVATION | - |
dc.subject | ANTIOXIDANT | - |
dc.subject | CELLS | - |
dc.title | AP-1-Targeted Inhibition of Macrophage Function and Lipopolysaccharide/D-Galactosamine-Induced Hepatitis by Phyllanthus acidus Methanolic Extract | - |
dc.type | Article | - |
dc.identifier.doi | 10.1142/S0192415X15500652 | - |
dc.type.rims | ART | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF CHINESE MEDICINE, v.43, no.6, pp.1137 - 1158 | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000362666600005 | - |
dc.identifier.scopusid | 2-s2.0-84943581715 | - |
dc.citation.endPage | 1158 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1137 | - |
dc.citation.title | AMERICAN JOURNAL OF CHINESE MEDICINE | - |
dc.citation.volume | 43 | - |
dc.contributor.affiliatedAuthor | Kim, Mi-Yeon | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Phyllanthus acidus (Phyllanthaceae) | - |
dc.subject.keywordAuthor | MAPK | - |
dc.subject.keywordAuthor | AP-1 | - |
dc.subject.keywordAuthor | TNF-alpha | - |
dc.subject.keywordAuthor | IL-1 beta | - |
dc.subject.keywordAuthor | IL-6 | - |
dc.subject.keywordAuthor | Hepatitis | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | ETHANOL EXTRACT | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | ANTIINFLAMMATORY ACTIVITIES | - |
dc.subject.keywordPlus | MOLECULAR-MECHANISMS | - |
dc.subject.keywordPlus | SIGNALING PATHWAYS | - |
dc.subject.keywordPlus | LIVER-INJURY | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | ANTIOXIDANT | - |
dc.subject.keywordPlus | CELLS | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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