자근의 성분 분리 및 이들 성분의 HPLC-UV 동시분석법 개발Isolation of Constituents of Lihtospermum erythrorhizon and Development of an Analytical Method of its Constituents by HPLC-UV
- Authors
- 홍은영; 경민지; 황완균
- Issue Date
- Oct-2015
- Publisher
- 대한약학회
- Keywords
- Lithospermi radix; acetylshikonin; β-hydroxyisovalerylshikonin; HPLC
- Citation
- 약 학 회 지, v.59, no.5, pp 222 - 229
- Pages
- 8
- Journal Title
- 약 학 회 지
- Volume
- 59
- Number
- 5
- Start Page
- 222
- End Page
- 229
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/10427
- ISSN
- 0377-9556
- Abstract
- Lithospermi radix, the dried roots of Litospermum erythrorhizon Sieboid et Zuccarini (Boraginaceae), has long been used to treat detoxification and inflammation. In this study, we isolated two main quinoid compounds, β-hydroxyisovalerylshikonin (1) and acetylshikonin (2) from L. erythrorhizon. As acetylshikonin is considered as a marker compound of L. erythrorhizon, a rapid analysis method for the simultaneous determination of quinoid compounds including 2 was also developed by HPLC (High Performance Liquid Chromatography) and validation of this analytical method. By the developed method, two quinoid marker compounds (β-hydroxyisovalerylshikonin and acetylshikonin) were successfully quantified in 31 commercial samples which were collected from different regions. The contents were 0.20% (β-hydroxyisovalerylshikonin) and 0.22% (acetylshikonin), respectively.
Lithospermi radix, the dried roots of Litospermum erythrorhizon Sieboid et Zuccarini (Boraginaceae), has long been used to treat detoxification and inflammation. In this study, we isolated two main quinoid compounds, β-hydroxyisovalerylshikonin (1) and acetylshikonin (2) from L. erythrorhizon. As acetylshikonin is considered as a marker compound of L. erythrorhizon, a rapid analysis method for the simultaneous determination of quinoid compounds including 2 was also developed by HPLC (High Performance Liquid Chromatography) and validation of this analytical method. By the developed method, two quinoid marker compounds (β-hydroxyisovalerylshikonin and acetylshikonin) were successfully quantified in 31 commercial samples which were collected from different regions. The contents were 0.20% (β-hydroxyisovalerylshikonin) and 0.22% (acetylshikonin), respectively.
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