Multiplex polymerase chain reaction assays for the detection of the zearalenone chemotype of Fusarium species in white and brown rice
DC Field | Value | Language |
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dc.contributor.author | Sim, Jae Ho | - |
dc.contributor.author | Tian, Fei | - |
dc.contributor.author | Jung, Soo Yeon | - |
dc.contributor.author | Auh, Joong-Hyuck | - |
dc.contributor.author | Chun, Hyang Sook | - |
dc.date.available | 2019-01-22T14:03:50Z | - |
dc.date.issued | 2018-03 | - |
dc.identifier.issn | 0168-1605 | - |
dc.identifier.issn | 1879-3460 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/1060 | - |
dc.description.abstract | Early detection of the zearalenone (ZEA) chemotype of Fusarium species could be a precautionary measure for preventing ZEA contamination in rice. In this study, a multiplex polymerase chain reaction (mPCR) assay for detecting ZEA-producing fungi in rice was established using a set of four primers targeting the ZEA biosynthesis genes PKS3, PKS13, ZEB1, and ZEB2. Two mPCR approaches were used: one that amplified the DNA obtained from Fusarium isolates (conventional method) and another that directly amplified the target DNA from rice samples without time-consuming DNA isolation (direct method). The two mPCR methods showed high sensitivity in detecting ZEA-producing species, with a detection limit of 1.25 pg/mu L. of genomic DNA and 102 and 103 spores/g of white and brown rice, respectively. Both methods were specific for ZEA-producing species and gave four band patterns. The application of the two mPCR methods to 51 Fusarium isolates and 41 rice samples revealed that 31% (16 of 51) and 24% (10 of 41) of the samples were contaminated with ZEA-producing species, respectively. The mPCR results were further evaluated using high-performance liquid chromatography; in general, the two methods yielded similar results. These findings indicate that both mPCR methods are suitable for the detection of ZEA-producing Fusarium species in white and brown rice; however, the direct method yielded more rapid results. | - |
dc.format.extent | 8 | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Multiplex polymerase chain reaction assays for the detection of the zearalenone chemotype of Fusarium species in white and brown rice | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.ijfoodmicro.2018.02.003 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, v.269, pp 120 - 127 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000428103700016 | - |
dc.identifier.scopusid | 2-s2.0-85041385689 | - |
dc.citation.endPage | 127 | - |
dc.citation.startPage | 120 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | - |
dc.citation.volume | 269 | - |
dc.type.docType | Article | - |
dc.publisher.location | 네델란드 | - |
dc.subject.keywordAuthor | Fusarium species | - |
dc.subject.keywordAuthor | Zearalenone chemotype | - |
dc.subject.keywordAuthor | Multiplex PCR | - |
dc.subject.keywordAuthor | Conventional and direct methods | - |
dc.subject.keywordAuthor | Rice | - |
dc.subject.keywordPlus | PERFORMANCE LIQUID-CHROMATOGRAPHY | - |
dc.subject.keywordPlus | POLYKETIDE SYNTHASE GENES | - |
dc.subject.keywordPlus | GIBBERELLA-ZEAE | - |
dc.subject.keywordPlus | SOUTH-KOREA | - |
dc.subject.keywordPlus | BY-PRODUCTS | - |
dc.subject.keywordPlus | ANIMAL FEED | - |
dc.subject.keywordPlus | GRAMINEARUM | - |
dc.subject.keywordPlus | WHEAT | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordPlus | MYCOTOXIN | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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