Caenispirillum humi sp nov., a bacterium isolated from the soil of Korean pine garden

Abstract

A novel bacterial strain MAH-8(T) was isolated from a soil sample of a Korean pine garden and was characterized using a polyphasic approach. Cells were Gram-staining negative, pinkish yellow colored, motile and vibrio-shaped. The strain was aerobic and catalase, oxidase positive, optimum growth temperature and pH were 28-30 A degrees C and 7.0, respectively. On the basis of 16S rRNA gene sequence analysis, strain MAH-8(T) belongs to the genus Caenispirillum and is most closely related to Caenispirillum bisanense KCTC 12839(T) (98.14%), Caenispirillum deserti KCTC 42064(T) (96.35%), and Caenispirillum salinarum JCM 17360(T) (95.76%). In DNA-DNA hybridization tests, the DNA relatedness between strain MAH-8(T) and its closest phylogenetic neighbor was below 45.0%. The DNA G + C content was 70.5 mol% and the predominant respiratory quinone was ubiquinone-10. Flexirubin-type pigments were present and the major cellular fatty acids were C-18:1 omega 7c/C-18:1 omega 6c, C-16:1 omega 7c/C-16:1 omega 6c and C-16:0. The results of DNA-DNA hybridization and genotypic analysis in combination with chemotaxonomic and physiological data demonstrated that strain MAH-8(T) represented a novel species within the genus Caenispirillum, for which the name Caenispirillum humi, is proposed. The type strain is MAH-8(T) (= KACC 19294(T) = CGMCC 1.16224(T)). The NCBI GenBank Accession Number for the 16S rRNA gene sequence of strain MAH-8(T) is KY964275.