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Quantitative analysis of (+)-catechin and diosgenin from Smilax china L. Rhizome
- Authors
- Yin, J.; Yoon, K.H.; Hwang, Y.J.; Lee, J.Y.; Shin, H.S.; Won Lee, M.
- Issue Date
- 2015
- Publisher
- Korean Society of Pharmacognosy
- Keywords
- (+)-Catechin; Acid hydrolysis; Content; Diosgenin; Smilax china L; UPLC; Validation
- Citation
- Korean Journal of Pharmacognosy, v.46, no.3, pp 189 - 194
- Pages
- 6
- Journal Title
- Korean Journal of Pharmacognosy
- Volume
- 46
- Number
- 3
- Start Page
- 189
- End Page
- 194
- ISSN
- 0253-3073
- Abstract
- Validation and contents determination of (+)-catechin and diosgenin from Smilax china L. rhizome (SC) were confirmed using Ultra-Performance Liquid Chromatography (UPLC). (+)-Catechin was isolated from 60% prethanol SC extract and diosgenin was isolated by acid hydrolysis of saponin fraction from 60% prethanol extract of SC. Finally we have established the validation of the isolated compounds [(+)-catechin, diosgenin] and contents determinations of (+)-catechin and diosgenin by UPLC on 60% prethanol extract of SC [(+)-catechin: 0.06878%, diosgenin: 0.48169%], and on hot water extract of SC [(+)-catechin: 0.06584%, diosgenin: 0.42178%]. © 2015, Korean Society of Pharmacognosy. All rights reserved.
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