Influence of cationic lipid concentration on properties of lipid-polymer hybrid nanospheres for gene delivery
DC Field | Value | Language |
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dc.contributor.author | Bose, Rajendran Jc | - |
dc.contributor.author | Arai, Yoshie | - |
dc.contributor.author | Ahn, Jong Chan | - |
dc.contributor.author | Park, Hansoo | - |
dc.contributor.author | Lee, Soo-Hong | - |
dc.date.available | 2019-03-08T20:40:05Z | - |
dc.date.issued | 2015-09-02 | - |
dc.identifier.issn | 1178-2013 | - |
dc.identifier.issn | 1178-2013 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/11466 | - |
dc.description.abstract | Nanoparticles have been widely used for nonviral gene delivery. Recently, cationic hybrid nanoparticles consisting of two different materials were suggested as a promising delivery vehicle. In this study, nanospheres with a poly(D,L-lactic-co-glycolic acid) (PLGA) core and cationic lipid shell were prepared, and the effect of cationic lipid concentrations on the properties of lipid polymer hybrid nanocarriers investigated. Lipid-polymer hybrid nanospheres (LPHNSs) were fabricated by the emulsion-solvent evaporation method using different concentrations of cationic lipids and characterized for size, surface charge, stability, plasmid DNA-binding capacity, cytotoxicity, and transfection efficiency. All LPHNSs had narrow size distribution with positive surface charges (zeta-potential 52-60 mV), and showed excellent plasmid DNA-binding capacity. In vitro cytotoxicity measurements with HEK293T, HeLa, HaCaT, and HepG2 cells also showed that LPHNSs exhibited less cytotoxicity than conventional transfection agents, such as Lipofectamine and polyethyleneimine-PLGA. As cationic lipid concentrations increased, the particle size of LPHNSs decreased while their zeta-potential increased. In addition, the in vitro transfection efficiency of LPHNSs increased as lipid concentration increased. | - |
dc.format.extent | 16 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | DOVE MEDICAL PRESS LTD | - |
dc.title | Influence of cationic lipid concentration on properties of lipid-polymer hybrid nanospheres for gene delivery | - |
dc.type | Article | - |
dc.identifier.doi | 10.2147/IJN.S87120 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF NANOMEDICINE, v.10, pp 5367 - 5382 | - |
dc.description.isOpenAccess | Y | - |
dc.identifier.wosid | 000387888900001 | - |
dc.identifier.scopusid | 2-s2.0-84980532351 | - |
dc.citation.endPage | 5382 | - |
dc.citation.startPage | 5367 | - |
dc.citation.title | INTERNATIONAL JOURNAL OF NANOMEDICINE | - |
dc.citation.volume | 10 | - |
dc.type.docType | Article | - |
dc.publisher.location | 뉴질랜드 | - |
dc.subject.keywordAuthor | core-shell hybrid nanospheres | - |
dc.subject.keywordAuthor | lipid concentration | - |
dc.subject.keywordAuthor | surface modification | - |
dc.subject.keywordAuthor | low cytotoxicity | - |
dc.subject.keywordAuthor | transfection efficiency | - |
dc.subject.keywordPlus | IN-VITRO PERFORMANCE | - |
dc.subject.keywordPlus | TRANSFECTION EFFICIENCY | - |
dc.subject.keywordPlus | CELLULAR UPTAKE | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | COMPLEXES | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | SHELL | - |
dc.subject.keywordPlus | SIRNA | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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