Detailed Information

Cited 23 time in webofscience Cited 24 time in scopus
Metadata Downloads

Influence of cationic lipid concentration on properties of lipid-polymer hybrid nanospheres for gene delivery

Full metadata record
DC Field Value Language
dc.contributor.authorBose, Rajendran Jc-
dc.contributor.authorArai, Yoshie-
dc.contributor.authorAhn, Jong Chan-
dc.contributor.authorPark, Hansoo-
dc.contributor.authorLee, Soo-Hong-
dc.date.available2019-03-08T20:40:05Z-
dc.date.issued2015-09-02-
dc.identifier.issn1178-2013-
dc.identifier.issn1178-2013-
dc.identifier.urihttps://scholarworks.bwise.kr/cau/handle/2019.sw.cau/11466-
dc.description.abstractNanoparticles have been widely used for nonviral gene delivery. Recently, cationic hybrid nanoparticles consisting of two different materials were suggested as a promising delivery vehicle. In this study, nanospheres with a poly(D,L-lactic-co-glycolic acid) (PLGA) core and cationic lipid shell were prepared, and the effect of cationic lipid concentrations on the properties of lipid polymer hybrid nanocarriers investigated. Lipid-polymer hybrid nanospheres (LPHNSs) were fabricated by the emulsion-solvent evaporation method using different concentrations of cationic lipids and characterized for size, surface charge, stability, plasmid DNA-binding capacity, cytotoxicity, and transfection efficiency. All LPHNSs had narrow size distribution with positive surface charges (zeta-potential 52-60 mV), and showed excellent plasmid DNA-binding capacity. In vitro cytotoxicity measurements with HEK293T, HeLa, HaCaT, and HepG2 cells also showed that LPHNSs exhibited less cytotoxicity than conventional transfection agents, such as Lipofectamine and polyethyleneimine-PLGA. As cationic lipid concentrations increased, the particle size of LPHNSs decreased while their zeta-potential increased. In addition, the in vitro transfection efficiency of LPHNSs increased as lipid concentration increased.-
dc.format.extent16-
dc.language영어-
dc.language.isoENG-
dc.publisherDOVE MEDICAL PRESS LTD-
dc.titleInfluence of cationic lipid concentration on properties of lipid-polymer hybrid nanospheres for gene delivery-
dc.typeArticle-
dc.identifier.doi10.2147/IJN.S87120-
dc.identifier.bibliographicCitationINTERNATIONAL JOURNAL OF NANOMEDICINE, v.10, pp 5367 - 5382-
dc.description.isOpenAccessY-
dc.identifier.wosid000387888900001-
dc.identifier.scopusid2-s2.0-84980532351-
dc.citation.endPage5382-
dc.citation.startPage5367-
dc.citation.titleINTERNATIONAL JOURNAL OF NANOMEDICINE-
dc.citation.volume10-
dc.type.docTypeArticle-
dc.publisher.location뉴질랜드-
dc.subject.keywordAuthorcore-shell hybrid nanospheres-
dc.subject.keywordAuthorlipid concentration-
dc.subject.keywordAuthorsurface modification-
dc.subject.keywordAuthorlow cytotoxicity-
dc.subject.keywordAuthortransfection efficiency-
dc.subject.keywordPlusIN-VITRO PERFORMANCE-
dc.subject.keywordPlusTRANSFECTION EFFICIENCY-
dc.subject.keywordPlusCELLULAR UPTAKE-
dc.subject.keywordPlusNANOPARTICLES-
dc.subject.keywordPlusCOMPLEXES-
dc.subject.keywordPlusCELLS-
dc.subject.keywordPlusPLATFORM-
dc.subject.keywordPlusTHERAPY-
dc.subject.keywordPlusSHELL-
dc.subject.keywordPlusSIRNA-
dc.relation.journalResearchAreaScience & Technology - Other Topics-
dc.relation.journalResearchAreaPharmacology & Pharmacy-
dc.relation.journalWebOfScienceCategoryNanoscience & Nanotechnology-
dc.relation.journalWebOfScienceCategoryPharmacology & Pharmacy-
dc.description.journalRegisteredClasssci-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
Files in This Item
Appears in
Collections
College of ICT Engineering > School of Integrative Engineering > 1. Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Park, Hansoo photo

Park, Hansoo
창의ICT공과대학 (융합공학부)
Read more

Altmetrics

Total Views & Downloads

BROWSE