Influence of cationic lipid concentration on properties of lipid-polymer hybrid nanospheres for gene deliveryopen access
- Authors
- Bose, Rajendran Jc; Arai, Yoshie; Ahn, Jong Chan; Park, Hansoo; Lee, Soo-Hong
- Issue Date
- 2-Sep-2015
- Publisher
- DOVE MEDICAL PRESS LTD
- Keywords
- core-shell hybrid nanospheres; lipid concentration; surface modification; low cytotoxicity; transfection efficiency
- Citation
- INTERNATIONAL JOURNAL OF NANOMEDICINE, v.10, pp 5367 - 5382
- Pages
- 16
- Journal Title
- INTERNATIONAL JOURNAL OF NANOMEDICINE
- Volume
- 10
- Start Page
- 5367
- End Page
- 5382
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/11466
- DOI
- 10.2147/IJN.S87120
- ISSN
- 1178-2013
1178-2013
- Abstract
- Nanoparticles have been widely used for nonviral gene delivery. Recently, cationic hybrid nanoparticles consisting of two different materials were suggested as a promising delivery vehicle. In this study, nanospheres with a poly(D,L-lactic-co-glycolic acid) (PLGA) core and cationic lipid shell were prepared, and the effect of cationic lipid concentrations on the properties of lipid polymer hybrid nanocarriers investigated. Lipid-polymer hybrid nanospheres (LPHNSs) were fabricated by the emulsion-solvent evaporation method using different concentrations of cationic lipids and characterized for size, surface charge, stability, plasmid DNA-binding capacity, cytotoxicity, and transfection efficiency. All LPHNSs had narrow size distribution with positive surface charges (zeta-potential 52-60 mV), and showed excellent plasmid DNA-binding capacity. In vitro cytotoxicity measurements with HEK293T, HeLa, HaCaT, and HepG2 cells also showed that LPHNSs exhibited less cytotoxicity than conventional transfection agents, such as Lipofectamine and polyethyleneimine-PLGA. As cationic lipid concentrations increased, the particle size of LPHNSs decreased while their zeta-potential increased. In addition, the in vitro transfection efficiency of LPHNSs increased as lipid concentration increased.
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