Growth Suppression of Colorectal Cancer by Plant-Derived Multiple mAb CO17-1A x BR55 via Inhibition of ERK1/2 Phosphorylation
- Authors
- Kwak, Dong Hoon; Moussavou, Ghislain; Lee, Ju Hyoung; Heo, Sung Youn; Ko, Kisung; Hwang, Kyung-A; Jekal, Seung-Joo; Choo, Young-Kug
- Issue Date
- Nov-2014
- Publisher
- MDPI AG
- Keywords
- anti-EpCAM, colon cancer; mAb(P) CO17-1A x BR5; apoptosis; mAb(P) CO17-1A; monoclonal antibody
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.15, no.11, pp 21105 - 21119
- Pages
- 15
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- Volume
- 15
- Number
- 11
- Start Page
- 21105
- End Page
- 21119
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/11684
- DOI
- 10.3390/ijms151121105
- ISSN
- 1422-0067
1422-0067
- Abstract
- We have generated the transgenic Tabaco plants expressing multiple monoclonal antibody (mAb) CO7-1A x BR55 by cross-pollinating with mAb CO17-1A and mAb BR55. We have demonstrated the anti-cancer effect of plant-derived multiple mAb CO17-1A x BR55. We find that co-treatment of colorectal mAbs (anti-epithelial cellular adhesion molecule (EpCAM), plant-derived monoclonal antibody (mAb(P)) CO17-1A and mAb(P) CO17-1A x BR55) with RAW264.7 cells significantly inhibited the cell growth in SW620 cancer cells. In particular, multi mAb(P) CO17-1A x BR55 significantly and efficiently suppressed the growth of SW620 cancer cells compared to another mAbs. Apoptotic death-positive cells were significantly increased in the mAb(P) CO17-1A x BR55-treated. The mAb(P) CO17-1A x BR55 treatment significantly decreased the expression of B-Cell lymphoma-2 (BCl-2), but the expression of Bcl-2-associated X protein (Bax), and cleaved caspase-3 were markedly increased. In vivo, the mAb(P) CO17-1A x BR55 significantly and efficiently inhibited the growth of colon tumors compared to another mAbs. The apoptotic cell death and inhibition of pro-apoptotic proteins expression were highest by treatment with mAb(P) CO17-1A x BR55. In addition, the mAb(P) CO17-1A x BR55 significantly inhibited the extracellular signal-regulated kinase 1 and 2 (ERK1/2) phosphorylation in cancer cells and tumors. Therefore, this study results suggest that multiple mAb(P) CO17-1A x BR55 has a significant effect on apoptosis-mediated anticancer by suppression of ERK1/2 phosphorylation in colon cancer compared to another mAbs. In light of these results, further clinical investigation should be conducted on mAb(P) CO17-1A x BR55 to determine its possible chemopreventive and/or therapeutic efficacy against human colon cancer.
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