Bacterial beta-(1,3)-glucan prevents DSS-induced IBD by restoring the reduced population of regulatory T cells
- Authors
- Lee, Kwang-Ho; Park, Min; Ji, Kon-Young; Lee, Hwa-Youn; Jang, Ji-Hun; Yoon, Il-Joo; Oh, Seung-Su; Kim, Su-Man; Jeong, Yun-Hwa; Yun, Chul-Ho; Kim, Mi-Kyoung; Lee, In-Young; Choi, Ha-Rim; Ko, Ki-sung; Kang, Hyung-Sik
- Issue Date
- Oct-2014
- Publisher
- ELSEVIER GMBH, URBAN & FISCHER VERLAG
- Keywords
- Bacterial beta-(1,3)-glucan; Inflammatory bowel disease; Dextran sulfate sodium (DSS); Regulatory T cell; Natural killer cell; Immunoglobulin A
- Citation
- IMMUNOBIOLOGY, v.219, no.10, pp 802 - 812
- Pages
- 11
- Journal Title
- IMMUNOBIOLOGY
- Volume
- 219
- Number
- 10
- Start Page
- 802
- End Page
- 812
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/11745
- DOI
- 10.1016/j.imbio.2014.07.003
- ISSN
- 0171-2985
1878-3279
- Abstract
- Bacterial beta-(1,3)-glucan has more advantages in terms of cost, yield and efficiency than that derived from mushrooms, plants, yeasts and fungi. We have previously developed a novel and high-yield beta-(1,3)glucan produced by Agrobacterium sp. R259. This study aimed to elucidate the functional mechanism and therapeutic efficacy of bacterial beta-(1,3)-glucan in dextran sulfate sodium (DSS)-induced inflammatory bowel disease (IBD).Mice were orally pretreated with bacterial beta-(1,3)-glucan at daily doses of 2.5 or 5 mg/kg for 2 weeks. After 6 days of DSS treatment, clinical assessment of IBD severity and expression of pro-inflammatory cytokines were evaluated. In vivo cell proliferation was examined by immunohistochemistry using Ki-67 and ER-TR7 antibodies. The frequency of regulatory T cells (Tregs) was analyzed by flow cytometry. Natural killer (NK) activity and IgA level were evaluated using NK cytotoxicity assay and ELISA.The deterioration of body weight gain, colonic architecture, disease score and histological score was recovered in DSS-induced IBD mice when pretreated with bacterial beta-(1,3)-glucan. The recruitment of macrophages and the gene expression of proinflammatory cytokines, such as IL-1 beta, IL-6 and IL-17A/F, were markedly decreased in the colon of beta-(1,3)-glucan-pretreated mice. beta-(1,3)-Glucan induced the recovery of Tregs in terms of their frequency in DSS-induced IBD mice. Intriguingly, beta-(1,3)-glucan reversed the functional defects of NK cells and excessive IgA production in DSS-induced IBD mice.We conclude that bacterial beta-(1,3)-glucan prevented the progression of DSS-induced IBD by recovering the reduction of Tregs, functional defect of NK cells and excessive IgA production. (C) 2014 Elsevier GmbH. All rights reserved.
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