UPLC-MS/MS Method for Determination of Bepotastine in Human Plasma
- Authors
- Choi, Yun Kyoung; Chung, Yoon Hee; Nam, Yun Sung; Kang, Da Young; Kim, Hohyun; Lee, Seo Eun; Kim, Hak Rim; Lee, Yong Seong; Jeong, Ji Hoon
- Issue Date
- Sep-2014
- Publisher
- OXFORD UNIV PRESS INC
- Citation
- JOURNAL OF CHROMATOGRAPHIC SCIENCE, v.52, no.8, pp 886 - 893
- Pages
- 8
- Journal Title
- JOURNAL OF CHROMATOGRAPHIC SCIENCE
- Volume
- 52
- Number
- 8
- Start Page
- 886
- End Page
- 893
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/11886
- DOI
- 10.1093/chromsci/bmt135
- ISSN
- 0021-9665
1945-239X
- Abstract
- A sensitive and rapid method for quantitation of bepotastine in human plasma has been established using ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS). Valsartan was used as an internal standard. Bepotastine and internal standard in plasma sample were extracted using ethylacetate (liquid-liquid extraction). A centrifuged upper layer was then evaporated and reconstituted with the mobile phase of acetonitrile-5 mM ammonium formate (pH 3.5) (85: 15, v/v). The reconstituted samples were injected into a phenyl column. Using MS/MS in the multiple reaction monitoring mode, bepotastine and valsartan were detected without severe interference from human plasma matrix. Bepotastine produced a protonated precursor ion ([M+H](+)) at m/z 389 and a corresponding product ion at m/z 167. And the internal standard produced a protonated precursor ion ([M+H](+)) at m/z 436 and a corresponding product ion at m/z 291. Detection of bepotastine in human plasma by the UPLC-ESI-MS/MS method was accurate and precise with a quantitation limit of 0.2 ng/mL. The validation, reproducibility, stability and recovery of the method were evaluated. The method has been successfully applied to pharmacokinetic studies of bepotastine in human plasma.
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