Flavihumibacter solisilvae sp nov., isolated from forest soil

Abstract

A Gram-stain-positive, strictly aerobic, yellow colony-forming bacterium, designated strain 3-3(T), was isolated from forest soil of Bac Kan Province in Vietnam. Cells were non-motile rods without gliding motility, showing oxidase- and catalase-positive reactions. Growth was observed at 20-37 degrees C (optimum, 28 degrees C) and pH 5.5-9.5 (optimum, pH 7.5). The major cellular fatty acids were iso-C-15:0, iso-C-15:1 G and summed feature 3 (comprising C-16:1 omega 6c and/or C-16:1 omega 7c). Strain 3-3T contained phosphatidylethanoamine, three unidentified aminolipids and three unidentified lipids. The G+C content of the genomic DNA was 49.5 mol% and the only isoprenoid quinone detected was menaquinone 7 (MK-7). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-3(T) formed a tight phylogenetic lineage with Flavihumibacter petaseus T41(T) with a bootstrap value of 100%. Strain 3-3(T) was related most closely to F. petaseus T41(T) with 97.3% 16S rRNA gene sequence similarity and the level of DNA DNA relatedness between the two was 9.4 +/- 1.2%. Based on phenotypic, chemotaxonomic and molecular features, strain 3-3T represents a novel species of the genus Flavihumibacter, for which the name Flavihumibacter solisilvae sp. nov. is proposed. The type strain is 3-3(T) (=KACC 17917(T)=JCM 19891(T)).