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Cited 14 time in webofscience Cited 14 time in scopus
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Selective transfection with osmotically active sorbitol modified PEI nanoparticles for enhanced anti-cancer gene therapy

Authors
Kim Cuc Thi NguyenMuthiah, MuthunarayananIslam, Mohammad ArifulKalash, R. SanthoshCho, Chong-SuPark, HansooLee, Il-KwonKim, Hyeoung-JoonPark, In-KyuCho, Kyung A.
Issue Date
Jul-2014
Publisher
ELSEVIER SCIENCE BV
Keywords
Transfection efficiency; Polyethylenimine; Sorbitol; Caveolae-dependent endocytosis
Citation
COLLOIDS AND SURFACES B-BIOINTERFACES, v.119, pp 126 - 136
Pages
11
Journal Title
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume
119
Start Page
126
End Page
136
URI
https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/12054
DOI
10.1016/j.colsurfb.2014.05.003
ISSN
0927-7765
1873-4367
Abstract
Polysorbitol-mediated transporter (PSMT) has been previously shown to achieve high transfection efficiency with minimal cytotoxicity. Polysorbitol backbone possesses osmotic properties and leads to enhanced cellular uptake. The PSMT/pDNA nanoparticles were prepared and the particle size, surface charge of the nanoparticles was determined for the study. PSMT delivers genes into cells by the caveolae mediated endocytic pathway. Caveolae expression is usually altered in transformed cancer cells. Transfection through the caveolae may help PSMT to selectively transfect cancer cells rather than normal cells. Transfection of the luciferase gene by PSMT was tested in various cell types including cancer cell lines, primary cells, and immortalized cells. Luciferase transgene expression mediated by PSMT was remarkably increased in HeLa cells compared to expression using the control carrier Lipofectamine. Moreover, the toxicity of PSMT was comparable to the control carrier (Lipofectamine) in the same cells. Selective transfection of cancer cells using PSMT was further confirmed by co-culture of cancer and normal cells, which showed that transgene expression was pre-dominantly achieved in cancer cells. A functional p53 gene was also delivered into HeLa cells using PSMT and the selective transgene expression of p53 protein in cancer cells was analyzed through western blotting and confocal microscopy. HeLa cells transfected with PSMT/p53 plasmid nanoparticles showed cellular damage and apoptosis, which was confirmed through propidium iodide staining. (C) 2014 Elsevier B.V. All rights reserved.
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