Inhibition of Ku70 acetylation by INHAT subunit SET/TAF-I beta regulates Ku70-mediated DNA damage response
- Authors
- Kim, Kee-Beom; Kim, Dong-Wook; Park, Jin Woo; Jeon, Young-Joo; Kim, Daehwan; Rhee, Sangmyung; Chae, Jung-Il; Seo, Sang-Beom
- Issue Date
- Jul-2014
- Publisher
- SPRINGER BASEL AG
- Keywords
- SET/TAF-I beta; Ku70; Ku80; Acetylation; DNA damage response
- Citation
- CELLULAR AND MOLECULAR LIFE SCIENCES, v.71, no.14, pp 2731 - 2745
- Pages
- 15
- Journal Title
- CELLULAR AND MOLECULAR LIFE SCIENCES
- Volume
- 71
- Number
- 14
- Start Page
- 2731
- End Page
- 2745
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/12080
- DOI
- 10.1007/s00018-013-1525-8
- ISSN
- 1420-682X
1420-9071
- Abstract
- DNA double-strand breaks (DSBs) can cause either cell death or genomic instability. The Ku heterodimer Ku70/80 is required for the NHEJ (non-homologous end-joining) DNA DSB repair pathway. The INHAT (inhibitor of histone acetyltransferases) complex subunit, SET/TAF-I beta, can inhibit p300- and PCAF-mediated acetylation of both histone and p53, thereby repressing general transcription and that of p53 target genes. Here, we show that SET/TAF-I beta interacts with Ku70/80, and that this interaction inhibits CBP- and PCAF-mediated Ku70 acetylation in an INHAT domain-dependent manner. Notably, DNA damage by UV disrupted the interaction between SET/TAF-I beta and Ku70. Furthermore, we demonstrate that overexpressed SET/TAF-I beta inhibits recruitment of Ku70/80 to DNA damage sites. We propose that dysregulation of SET/TAF-I beta expression prevents repair of damaged DNA and also contributes to cellular proliferation. All together, our findings indicate that SET/TAF-I beta interacts with Ku70/80 in the nucleus and inhibits Ku70 acetylation. Upon DNA damage, SET/TAF-I beta dissociates from the Ku complex and releases Ku70/Ku80, which are then recruited to DNA DSB sites via the NHEJ DNA repair pathway.
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