Infectivity and complete nucleotide sequence of cucumber fruit mottle mosaic virus isolate Cm cDNA
DC Field | Value | Language |
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dc.contributor.author | Rhee, Sun-Ju | - |
dc.contributor.author | Hong, Jin-Sung | - |
dc.contributor.author | Lee, Gung Pyo | - |
dc.date.available | 2019-03-08T21:40:48Z | - |
dc.date.issued | 2014-07 | - |
dc.identifier.issn | 0304-8608 | - |
dc.identifier.issn | 1432-8798 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/12081 | - |
dc.description.abstract | Three isolates of cucumber fruit mottle mosaic virus (CFMMV) were collected from melon, cucumber, and pumpkin plants in Korea. A full-length cDNA clone of CFMMV-Cm (melon isolate) was produced and evaluated for infectivity after T7 transcription in vitro (pT7CF-Cm-flc). The complete CFMMV genome sequence of the infectious clone pT7CF-Cm-flc was determined. The genome of CFMMV-Cm consisted of 6,571 nucleotides and shared high nucleotide sequence identity (98.8 %) with the Israel isolate of CFMMV. Based on the infectious clone pT7CF-Cm-flc, a CaMV 35S-promoter driven cDNA clone (p35SCF-Cm-flc) was subsequently constructed and sequenced. Mechanical inoculation with RNA transcripts of pT7CF-Cm-flc and agro-inoculation with p35SCF-Cm-flc resulted in systemic infection of cucumber and melon, producing symptoms similar to those produced by CFMMV-Cm. Progeny virus in infected plants was detected by RT-PCR, western blot assay, and transmission electron microscopy. | - |
dc.format.extent | 5 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER WIEN | - |
dc.title | Infectivity and complete nucleotide sequence of cucumber fruit mottle mosaic virus isolate Cm cDNA | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s00705-014-1990-x | - |
dc.identifier.bibliographicCitation | ARCHIVES OF VIROLOGY, v.159, no.7, pp 1807 - 1811 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000338517000029 | - |
dc.identifier.scopusid | 2-s2.0-84903610273 | - |
dc.citation.endPage | 1811 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1807 | - |
dc.citation.title | ARCHIVES OF VIROLOGY | - |
dc.citation.volume | 159 | - |
dc.type.docType | Article | - |
dc.publisher.location | 오스트리아 | - |
dc.subject.keywordAuthor | Cucumis melo L. | - |
dc.subject.keywordAuthor | Cucumis sativus L. | - |
dc.subject.keywordAuthor | Full-length cDNA | - |
dc.subject.keywordAuthor | In vitro transcription | - |
dc.subject.keywordAuthor | Agro-inoculation | - |
dc.subject.keywordPlus | MOLECULAR CHARACTERIZATION | - |
dc.subject.keywordPlus | TOBAMOVIRUS | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | TOMATO | - |
dc.subject.keywordPlus | GENERATION | - |
dc.relation.journalResearchArea | Virology | - |
dc.relation.journalWebOfScienceCategory | Virology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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