Infectivity and complete nucleotide sequence of cucumber fruit mottle mosaic virus isolate Cm cDNA
- Authors
- Rhee, Sun-Ju; Hong, Jin-Sung; Lee, Gung Pyo
- Issue Date
- Jul-2014
- Publisher
- SPRINGER WIEN
- Keywords
- Cucumis melo L.; Cucumis sativus L.; Full-length cDNA; In vitro transcription; Agro-inoculation
- Citation
- ARCHIVES OF VIROLOGY, v.159, no.7, pp 1807 - 1811
- Pages
- 5
- Journal Title
- ARCHIVES OF VIROLOGY
- Volume
- 159
- Number
- 7
- Start Page
- 1807
- End Page
- 1811
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/12081
- DOI
- 10.1007/s00705-014-1990-x
- ISSN
- 0304-8608
1432-8798
- Abstract
- Three isolates of cucumber fruit mottle mosaic virus (CFMMV) were collected from melon, cucumber, and pumpkin plants in Korea. A full-length cDNA clone of CFMMV-Cm (melon isolate) was produced and evaluated for infectivity after T7 transcription in vitro (pT7CF-Cm-flc). The complete CFMMV genome sequence of the infectious clone pT7CF-Cm-flc was determined. The genome of CFMMV-Cm consisted of 6,571 nucleotides and shared high nucleotide sequence identity (98.8 %) with the Israel isolate of CFMMV. Based on the infectious clone pT7CF-Cm-flc, a CaMV 35S-promoter driven cDNA clone (p35SCF-Cm-flc) was subsequently constructed and sequenced. Mechanical inoculation with RNA transcripts of pT7CF-Cm-flc and agro-inoculation with p35SCF-Cm-flc resulted in systemic infection of cucumber and melon, producing symptoms similar to those produced by CFMMV-Cm. Progeny virus in infected plants was detected by RT-PCR, western blot assay, and transmission electron microscopy.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - ETC > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/12081)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.