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Sulforaphane induces reactive oxygen species-mediated mitotic arrest and subsequent apoptosis in human bladder cancer 5637 cells

Authors
Park, Hyun SooHan, Min HoKim, Gi-YoungMoon, Sung-KwonKim, Wun-JaeHwang, Hye JinPark, Kun YoungChoi, Yung Hyun
Issue Date
Feb-2014
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Keywords
Sulforaphane; ROS; Mitotic arrest; Apoptosis; 5637 Cells
Citation
FOOD AND CHEMICAL TOXICOLOGY, v.64, pp 157 - 165
Pages
9
Journal Title
FOOD AND CHEMICAL TOXICOLOGY
Volume
64
Start Page
157
End Page
165
URI
https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/12531
DOI
10.1016/j.fct.2013.11.034
ISSN
0278-6915
1873-6351
Abstract
The present study was undertaken to determine whether sulforaphane-derived reactive oxygen species (ROS) might cause growth arrest and apoptosis in human bladder cancer 5637 cells. Our results show that the reduced viability of 5637 cells by sulforaphane is due to mitotic arrest, but not the G2 phase. The sulforaphane-induced mitotic arrest correlated with an induction of cyclin B1 and phosphorylation of Cdk1, as well as a concomitant increased complex between cyclin B1 and Cdk1. Sulforaphane-induced apoptosis was associated with the activation of caspase-8 and -9, the initiators caspases of the extrinsic and intrinsic apoptotic pathways, respectively, and activation of effector caspase-3 and cleavage of poly (ADP-ribose) polymerase. However, blockage of caspase activation inhibited apoptosis and abrogated growth inhibition in sulforaphane-treated 5637 cells. This study further investigated the roles of ROS with respect to mitotic arrest and the apoptotic effect of sulforaphane, and the maximum level of ROS accumulation was observed 3 h after sulforaphane treatment. However, a ROS scavenger, N-acetyl-L-cysteine, notably attenuated sulforaphane-mediated apoptosis as well as mitotic arrest. Overall, these results suggest that sulforaphane induces mitotic arrest and apoptosis of 5637 cells via a ROS-dependent pathway. (C) 2013 Elsevier Ltd. All rights reserved.
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Moon, Sung Kwon
생명공학대학 (식품영양)
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