Bcl-2 Knockdown Accelerates T Cell Receptor-Triggered Activation-Induced Cell Death in Jurkat T Cells
- Authors
- Lee, Yun-Jung; Won, Tae Joon; Hyung, Kyeong Eun; Lee, Mi Ji; Moon, Young-hye; Lee, Ik Hee; Go, Byung Sung; Hwang, Kwang Woo
- Issue Date
- Feb-2014
- Publisher
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Keywords
- AICD; Apoptosis; Bcl-2; shRNA; T cell
- Citation
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, v.18, no.1, pp 73 - 78
- Pages
- 6
- Journal Title
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Volume
- 18
- Number
- 1
- Start Page
- 73
- End Page
- 78
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/12536
- DOI
- 10.4196/kjpp.2014.18.1.73
- ISSN
- 1226-4512
2093-3827
- Abstract
- Cell death and survival are tightly controlled through the highly coordinated activation/inhibition of diverse signal transduction pathways to insure normal development and physiology. Imbalance between cell death and survival often leads to autoimmune diseases and cancer. Death receptors sense extracellular signals to induce caspase-mediated apoptosis. Acting upstream of CED-3 family proteases, such as caspase-3, Bcl-2 prevents apoptosis. Using short hairpin RNAs (shRNAs), we suppressed Bcl-2 expression in Jurkat T cells, and this increased TCR-triggered AICD and enhanced TNFR gene expression. Also, knockdown of Bcl-2 in Jurkat T cells suppressed the gene expression of FLIP, TNF. receptor-associated factors 3 (TRAF3) and TRAF4. Furthermore, suppressed Bcl-2 expression increased caspase-3 and diminished nuclear factor kappa B (NF-kappa B) translocation.
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Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
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