소리쟁이 잎과 뿌리 성분 분석 및 사람 조골 유사 MG-63 세포 분화에 미치는 효과 비교

Abstract

Rumex crispus (curled dock), which is a perennial wild plant, has long been used as a laxative, astringent, andmedicine to treat blood and skin diseases. We recently reported that the roots of R. crispus are an effective nutraceuticalfor bone. This study prepared ethanol extracts of the leaves and roots of R. crispus, and analyzed the major constituentsusing liquid chromatography and mass spectrometry. In addition, their effects on the proliferation and differentiation ofhuman osteoblast-like MG-63 cells, such as cell viability, alkaline phosphatase (ALP) activity, collagen content, and mineralization,were compared. The chromatograms of the chemical constituents of the two extracts exhibited quite differentprofiles: quercetin and quercitrin were identified as major peaks in the leaf extract, whereas cinnamtannin B1 and procyanidinisomers were the major peaks for the root extract. Neither extract was cytotoxic at concentrations of <25 μg/ml. ALP activity and collagen synthesis—which are markers of the early stage of osteogenesis—in MG-63 cells were significantlyincreased upon the addition of the root extract compared with the addition of the leaf extract. In contrast, the leafextract had a more stimulatory effect on mineralization—which is marker of the late stage of osteogenesis—in MG-63 cellsthan did the root extract. In conclusion, extracts of both leaves and roots of R. crispus stimulated the bone-forming activityof osteoblasts; in particular, the root extract was more effective in the early stage of osteoblast differentiation, while the leafextract was more effective in the late stage. This difference in anabolic activity may be due to differences in the constituentsof the leaves and roots. The leaves and roots of R. crispus appear to complement each other as stimulators of bone formation.

Rumex crispus (curled dock), which is a perennial wild plant, has long been used as a laxative, astringent, andmedicine to treat blood and skin diseases. We recently reported that the roots of R. crispus are an effective nutraceuticalfor bone. This study prepared ethanol extracts of the leaves and roots of R. crispus, and analyzed the major constituentsusing liquid chromatography and mass spectrometry. In addition, their effects on the proliferation and differentiation ofhuman osteoblast-like MG-63 cells, such as cell viability, alkaline phosphatase (ALP) activity, collagen content, and mineralization,were compared. The chromatograms of the chemical constituents of the two extracts exhibited quite differentprofiles: quercetin and quercitrin were identified as major peaks in the leaf extract, whereas cinnamtannin B1 and procyanidinisomers were the major peaks for the root extract. Neither extract was cytotoxic at concentrations of <25 μg/ml. ALP activity and collagen synthesis—which are markers of the early stage of osteogenesis—in MG-63 cells were significantlyincreased upon the addition of the root extract compared with the addition of the leaf extract. In contrast, the leafextract had a more stimulatory effect on mineralization—which is marker of the late stage of osteogenesis—in MG-63 cellsthan did the root extract. In conclusion, extracts of both leaves and roots of R. crispus stimulated the bone-forming activityof osteoblasts; in particular, the root extract was more effective in the early stage of osteoblast differentiation, while the leafextract was more effective in the late stage. This difference in anabolic activity may be due to differences in the constituentsof the leaves and roots. The leaves and roots of R. crispus appear to complement each other as stimulators of bone formation.