Functional and Proteomic Alterations of F1 Capacitated Spermatozoa of Adult Mice Following Gestational Exposure to Bisphenol A
- Authors
- Rahman, Md Saidur; Kwon, Woo-Sung; Ryu, Do-Yeal; Khatun, Amena; Karmakar, Polash Chandra; Ryu, Buom-Yong; Pang, Myung-Geol
- Issue Date
- Jan-2018
- Publisher
- AMER CHEMICAL SOC
- Keywords
- bisphenol A; spermatozoa; capacitation; sperm function; fertility; proteomics
- Citation
- JOURNAL OF PROTEOME RESEARCH, v.17, no.1, pp 524 - 535
- Pages
- 12
- Journal Title
- JOURNAL OF PROTEOME RESEARCH
- Volume
- 17
- Number
- 1
- Start Page
- 524
- End Page
- 535
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/1370
- DOI
- 10.1021/acs.jproteome.7b00668
- ISSN
- 1535-3893
1535-3907
- Abstract
- Studies regarding bisphenol A (BPA) exposure and male (in)fertility have conventionally focused on modifications in ejaculated spermatozoa function from exposed individuals. However, mammalian spermatozoa are incapable of fertilization prior to achieving capacitation, the penultimate step in maturation. Therefore, it is necessary to investigate BPA-induced changes in capacitated spermatozoa and assess the consequences on subsequent fertilization. Here, we demonstrate the effect of gestational BPA exposure (50 mu g/kg bw/day, 5 mg/kg bw/day, and SO mg/kg bw/day) on the functions, biochemical properties, and proteomic profiles of F1 capacitated spermatozoa from adult mice. The data showed that high concentrations of BPA inhibited motility, motion kinematics, and capacitation of spermatozoa, perhaps because of increased lipid peroxidation and protein tyrosine nitration, and decreased intracellular ATP levels and protein kinase-A activity in spermatozoa. We also found that BPA compromised the rates of fertilization and early embryonic development. Differentially expressed proteins identified between BPA-exposed and control groups play a critical role in energy metabolism, stress responses, and fertility. Protein function abnormalities were responsible for the development of several diseases according to bioinformatics analysis. On the basis of these results, gestational exposure to BPA may alter capacitated spermatozoa function and the proteomic profile, ultimately affecting their fertility potential.
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