Performance of Anyplex (TM) II multiplex real-time PCR for the diagnosis of seven sexually transmitted infections: comparison with currently available methods
- Authors
- Choe, Hyun-Sop; Lee, Dong Sup; Lee, Seung-Ju; Hong, Sung-Hoo; Park, Dong Choon; Lee, Mi-Kyung; Kim, Tae-Hyoung; Cho, Yong-Hyun
- Issue Date
- Dec-2013
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Diagnosis; Sexually transmitted infection; Bacterial and parasite infection; Multiplex real-time PCR
- Citation
- INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, v.17, no.12, pp E1134 - E1140
- Journal Title
- INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
- Volume
- 17
- Number
- 12
- Start Page
- E1134
- End Page
- E1140
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14074
- DOI
- 10.1016/j.ijid.2013.07.011
- ISSN
- 1201-9712
1878-3511
- Abstract
- Objectives: The real-time PCR assay is the most sensitive test for screening and diagnosing sexually transmitted infections (STIs) and has made diagnosing these infections easier for clinicians. The aim of this study was to investigate the reliability, accuracy, and usefulness of the real-time multiplex PCR assay for the detection of seven sexually transmitted microorganisms in clinical samples. Methods: A total of 897 specimens from 365 symptomatic patients and 532 asymptomatic volunteers were collected over a 10-month period. A total of 696 subjects provided 50 ml of first-voided urine as samples, and 201 female patients provided endocervical swab specimens. Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum were tested for using five diagnostic methods: multiplex real-time PCR (Anyplex (TM) II), multiplex PCR (Seeplex (R)), strand displacement amplification (SDA, BD ProbeTec (TM) ET), PCR (AmpliSens (R)), and a commercially available Mycoplasma IST 2 Kit. Results: Multiplex real-time PCR (Anyplex (TM) II) showed outstanding results in all fields, particularly sensitivity and specificity, compared with other diagnostic tools. This method yielded 100% sensitivity and high specificity for the detection of C. trachomatis, N. gonorrhoeae, T. vaginalis, M. genitalium, and M. hominis. It was also useful for discriminating between U. urealyticum and U. parvum. Conclusions: Multiplex real-time PCR was found to be an equivalent or superior modality for the diagnosis of STIs. It could be a cost-effective and rapid diagnostic tool for the simultaneous detection of multiple STI microorganisms. (C) 2013 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > College of Medicine > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14074)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.