The Logic and Mechanism of Homologous Recombination Partner Choiceopen access
- Authors
- Hong, Soogil; Sung, Youngjin; Yu, Mi; Lee, Minsu; Kleckner, Nancy; Kim, Keun Pil
- Issue Date
- Aug-2013
- Publisher
- CELL PRESS
- Citation
- MOLECULAR CELL, v.51, no.4, pp 440 - 453
- Pages
- 14
- Journal Title
- MOLECULAR CELL
- Volume
- 51
- Number
- 4
- Start Page
- 440
- End Page
- 453
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14374
- DOI
- 10.1016/j.molcel.2013.08.008
- ISSN
- 1097-2765
1097-4164
- Abstract
- Recombinational repair of spontaneous double-strand breaks (DSBs) exhibits sister bias. DSB-initiated meiotic recombination exhibits homolog bias. Physical analysis in yeast reveals that, in both cases, the recombination reaction intrinsically gives homolog bias. From this baseline default, cohesin intervenes to confer sister bias, likely independent of cohesion. In meiosis, cohesin's sister-biasing effect is counteracted by RecA homolog Rad51 and its mediators, plus meiotic RecA homolog Dmc1, which thereby restore intrinsic homolog bias. Meiotic axis complex Red1/Mek1/Hopi participates by cleanly switching recombination from mitotic to meiotic mode, concomitantly activating Dmc1. We propose that a Rad51/DNA filament at one DSB end captures the intact sister, creating an anchor pad. This filament extends across the DSB site on the intact partner, precluding intersister strand exchange, thus forcing use of the homolog. Cohesin and Dmc1 interactively modulate this extension, with program-appropriate effects. In accord with this model, Rad51-mediated recombination in vivo requires the presence of a sister.
- Files in This Item
-
- Appears in
Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14374)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.