Antibacterial activity and protective effect against gastric cancer by Anthriscus sylvestris fractions

Abstract

The aim of this research was to investigate the industrial application of Anthriscus sylvestris. Antibacterial activities of the n-hexane, methylene chloride (MC), ethyl acetate, and butanol fractions of A. sylvestris were tested against Escherichia coli, Staphylococcus aureus, and Helicobacter pylori. The n-hexane and MC fractions showed a stronger antibacterial activity against S. aureus than other fractions, with an inhibition zone greater than 11 mm in disc assays. At 100 mu g center dot mL(-1), all fractions extracted from A. sylvestris scavenged more than 85% of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radicals (center dot OH); however, the n-hexane and MC fractions showed the strongest scavenging activity of DPPH and center dot OH. In addition, we found that treatment with the n-hexane and MC fractions inhibited the growth of H. pylori and gastric adenocarcinoma cells. Further testing of falcarindiol and deoxypodophyllotoxin from the MC fraction of A. sylvestris revealed the antibacterial effects against E. coli, S. aureus, and H. pylori, suggesting their potential use as antibacterial agents.