CRP detection from serum for chip-based point-of-care testing system
- Authors
- Kim, Chang-Hoon; Ahn, Jae-Hyuk; Kim, Jee-Yeon; Choi, Ji-Min; Lim, Kyung-Choon; Park, Tae Jung; Heo, Nam Su; Lee, Hee Gu; Kim, Jong-Wan; Choi, Yang-Kyu
- Issue Date
- Mar-2013
- Publisher
- ELSEVIER ADVANCED TECHNOLOGY
- Keywords
- C-reactive protein; CRP; Nanogap-embedded FET; FET-based biosensor; Point-of-care testing (POCT)
- Citation
- BIOSENSORS & BIOELECTRONICS, v.41, no.1, pp 322 - 327
- Pages
- 6
- Journal Title
- BIOSENSORS & BIOELECTRONICS
- Volume
- 41
- Number
- 1
- Start Page
- 322
- End Page
- 327
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14770
- DOI
- 10.1016/j.bios.2012.08.047
- ISSN
- 0956-5663
1873-4235
- Abstract
- Most of point-of-care testing (POCT) to improve facilitates in diagnosis, treatment, and monitoring of patients. POCT technique has still remained a quantitatively and accurately detective effect. In this article, we demonstrated that real human C-reactive protein (CRP) in serum was detected for a chip-based point-of-care testing application based on a nanogap-embedded field effect transistor (FET), and the results were compared with those obtained via the enzyme-linked immunosorbent assay (ELISA) method. The limit of detection (LOD), determined from the standard curve, was 0.1 ng/ml, which is comparable to that of commercialized ELISAs. We evaluated that an improved detection range (0.1 ng/ml to 100 ng/ml) was achieved by comparing with commercialized ELISA. Control experiments to determine selectivity and to discern false-positive/false-negative rates were also performed. This report is the first description of the detection of CRP in human serum using a silicon-based biosensor. (C) 2012 Elsevier B.V. All rights reserved.
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Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
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