Simultaneous Detection of Streptococcus pneumoniae, S. mitis, and S. oralis by a Novel Multiplex PCR Assay Targeting the gyrB Geneopen access
- Authors
- Kim, Wonyong; Park, Hee Kuk; Hwang, Woo-Jin; Shin, Hyoung-Shik
- Issue Date
- Mar-2013
- Publisher
- AMER SOC MICROBIOLOGY
- Citation
- JOURNAL OF CLINICAL MICROBIOLOGY, v.51, no.3, pp 835 - 840
- Pages
- 6
- Journal Title
- JOURNAL OF CLINICAL MICROBIOLOGY
- Volume
- 51
- Number
- 3
- Start Page
- 835
- End Page
- 840
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14791
- DOI
- 10.1128/JCM.02920-12
- ISSN
- 0095-1137
1098-660X
- Abstract
- A multiplex PCR (mPCR) protocol was developed for simultaneous detection of the gyrB gene in Streptococcus pneumoniae, Streptococcus mitis, and Streptococcus oralis, and the specificity was evaluated using 141 coccus strains. Genomic DNAs purified from S. pneumoniae, S. mitis, and S. oralis strains were efficiently detected with size differences, whereas no PCR products were amplified from any of the reference strains tested. A pilot study of 47 human oral swab specimens was conducted in parallel, and the mPCR assay identified S. pneumoniae in 1 sample, S. mitis in 8 samples, and S. oralis in 2 samples, providing a powerful means for characterization at the level of species compared with traditional culture analysis. Our results suggest that the mPCR protocol presented here is a sensitive and promising tool for the rapid detection and discrimination of S. pneumoniae, S. mitis, and S. oralis from clinical specimens.
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