Determination of robenidine residues in chicken muscle by high performance liquid chromatography with ultraviolet detection
- Authors
- Yeom, Hyesun; Yang, Dong-Hyug; Suh, Joon Hyuk; Eom, Han Young; Kim, Unyong; Kim, Junghyun; Lee, Seul Gi; Cho, Hyun-Deok; Han, Sang Beom
- Issue Date
- Mar-2013
- Publisher
- PHARMACEUTICAL SOC KOREA
- Keywords
- Robenidine; Residues; Chicken muscle; HPLC
- Citation
- ARCHIVES OF PHARMACAL RESEARCH, v.36, no.3, pp 359 - 365
- Pages
- 7
- Journal Title
- ARCHIVES OF PHARMACAL RESEARCH
- Volume
- 36
- Number
- 3
- Start Page
- 359
- End Page
- 365
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14824
- DOI
- 10.1007/s12272-013-0065-y
- ISSN
- 0253-6269
1976-3786
- Abstract
- A simple, robust and reliable method for the determination of residual robenidine in chicken muscle using high performance liquid chromatography with ultraviolet (UV) detection was developed and validated according to the Codex Alimentarius Commission guidelines. Chicken muscle was extracted by acetonitrile/formic acid (98:2, v/v) and defatted with hexane. Analytes were isocratically separated on a Luna C18 column (4.6 x 150 mm, 5 mu m) using 70 % methanol in water containing 0.1 % trifluoroacetic acid at a flow rate of 1.0 mL/min at 30 A degrees C. UV detection was performed at 312 nm. The method was validated by assessing performance parameters including selectivity, linearity, limit of quantification (LOQ), precision, accuracy, recovery, stability and robustness. A calibration curve that was constructed over 0.05-0.5 mu g/g showed correlation coefficients of more than 0.999. The intra- and inter-day precisions (as coefficient of variation) were 1.45-3.32 and 2.63-4.99 %, respectively. The intra- and inter-day accuracies were 99.4-105.3 and 98.3-101.6 %, respectively. The recoveries were in the range of 76.6-81.8 % and the LOQ was 0.05 mu g/g. The developed method showed suitable performance for the determination of robenidine residues in chicken muscle.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14824)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.