Real-Time Monitoring In Vitro Cellular Cytotoxicity of Silica Nanotubes Using Electric Cell-Substrate Impedance Sensing (ECIS)
- Authors
- Trong Binh Tran; Phuong Diem Nguyen; Um, Soong Ho; Son, Sang Jun; Min, Junhong
- Issue Date
- Feb-2013
- Publisher
- AMER SCIENTIFIC PUBLISHERS
- Keywords
- Electrical Measurement; ECIS; Cell Viability; Cytotoxicity; Silica; SNT; SNP; Micro Electrode
- Citation
- JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, v.9, no.2, pp 286 - 290
- Pages
- 5
- Journal Title
- JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
- Volume
- 9
- Number
- 2
- Start Page
- 286
- End Page
- 290
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14883
- DOI
- 10.1166/jbn.2013.1500
- ISSN
- 1550-7033
1550-7041
- Abstract
- An electrical measurement known as Electric Cell-substrate Impedance Sensing (ECIS) has become increasingly applied to the study of cellular viability, proliferation and cytotoxicity with the advantages of label-free, non-invasion and real-time monitoring capability in comparison with other conventional methods (MTS, MTT). With this technique, cells are grown on the micro-sized gold electrodes where the micro-ampere alternative current is applied to measure the impedance changes due to the physiological changes caused by internal or external stimuli. In another field, Silica Nanotubes (SNTs) are a novel class of inorganic structures with promising potentials in bio-separation, drug delivery, imaging and other biomedical applications. In this study, by using ECIS-based self-fabricated cell chip, Cells were cultured on the working electrodes and separately exposure to the 0, 2 mu m, 2 mu m and 10 mu m long at the varying concentrations of SNTs to evaluate the cellular responses such as viability, multiplication time and cytotoxicity. Final results were additionally compared with the MTS method as a reference to review the reliability
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