Voltage-dependent anion channels are a key factor of male fertility
- Authors
- Kwon, Woo-Sung; Park, Yoo-Jin; Mohamed, El-Sayed A.; Pang, Myung-Geol
- Issue Date
- Feb-2013
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- VDAC; DIDS; sperm function; male fertility
- Citation
- FERTILITY AND STERILITY, v.99, no.2, pp 354 - 361
- Pages
- 8
- Journal Title
- FERTILITY AND STERILITY
- Volume
- 99
- Number
- 2
- Start Page
- 354
- End Page
- 361
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/14890
- DOI
- 10.1016/j.fertnstert.2012.09.021
- ISSN
- 0015-0282
1556-5653
- Abstract
- Objective: To examine how voltage-dependent anion channels (VDACs) regulate sperm function in capacitation conditions. Design: Experimental prospective study. Setting: Academic research laboratory. Animal(s): Male ICR and female B6D2F1/CrljOri mice (8-12 weeks old). Intervention(s): Female mice were superovulated with 5 IU of pregnant mare serum gonadotropin given IP and 5 IU of hCG given IP 48 hours later. Oocytes were applied to assess fertilization and embryo development. Main Outcome Measure(s): Immunofluorescence assay, computer-assisted sperm analysis, hypo-osmotic swelling test, combined Hoechst 33258/chlortetracycline fluorescence assessment of capacitation status, measurement of [Ca2+](i) and [pH](i), Western blotting, and IVF. Result(s): VDAC2 was localized on the acrosomal region and principal piece, while VDAC3 was localized on the acrosomal region and midpiece. Blocking VDAC with DIDS (500 mu M) significantly decreased motility, viability, acrosome reaction, capacitation, tyrosine phosphorylation, fertilization, and embryo development regardless of Ca2+. However, the most severe decreases were observed in the presence (+) of DIDS and absence (-) of Ca2+, respectively. A significant decrease in [Ca2+](i) concentration was observed in (-) DIDS, while [pH](i) was significantly increased in (-) DIDS regardless of Ca2+. However, a significantly elevated [pH](i) was observed in (+) Ca2+. Conclusion(s): Abnormal regulation of VDACs negatively affected sperm function. Thus, VDACs may be key regulators of the fertilization ability of spermatozoa. (Fertil Steril (R) 2013;99:354-61. (C) 2013 by American Society for Reproductive Medicine.)
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