Simultaneous determination of polymethoxyflavones in Citrus species, Kiyomi tangor and Satsuma mandarin, by high performance liquid chromatography

Abstract

The content of polymethoxyflavones (PMFs) in Kiyomi tangor (Citrus unshiu Marcov. forma miyagawa-wase x C. sinensis) and Satsuma mandarin (C. unshiu Marcov. forma miyagawa-wase) was determined by HPLC/UV. The major PMFs of Kiyomi tangor were determined as 3,6,7,4'-tetramethoxyflavone in the peels (10.1 mg/g) and leaves (9.2 mg/g), and 3-hydroxy-5,6,7,4'-tetramethoxyflavone in the stems (1.5 mg/g). The major PMFs of Satsuma mandarin were determined as 5,6,7,8,4'-pentamethoxyflavone in the peels (2.2 mg/g) and leaves (1.6 mg/g), and 5,6,7,3',4'-pentamethoxyflavone in the stems (1.4 mg/g). Large amounts of Citrus by-products can ultimately provide a cheap and convenient source of PMFs. (C) 2012 Elsevier Ltd. All rights reserved.