LINE-1 hypomethylation is associated with radiation-induced genomic instability in industrial radiographers
- Authors
- Cho, Yoon Hee; Jang, Yoonhee; Woo, Hae Dong; Kim, Yang Jee; Kim, Su Young; Christensen, Sonja; Cole, Elizabeth; Choi, Soo Yong; Chung, Hai Won
- Issue Date
- Mar-2019
- Publisher
- WILEY
- Keywords
- global DNA hypomethylation; radiation; radiation-induced genomic instability; radiographers; LINE-1
- Citation
- ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, v.60, no.2, pp 174 - 184
- Pages
- 11
- Journal Title
- ENVIRONMENTAL AND MOLECULAR MUTAGENESIS
- Volume
- 60
- Number
- 2
- Start Page
- 174
- End Page
- 184
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/18157
- DOI
- 10.1002/em.22237
- ISSN
- 0893-6692
1098-2280
- Abstract
- Global DNA hypomethylation is proposed as a potential biomarker for cancer risk associated with genomic instability, which is an important factor in radiation-induced cancer. However, the associations among radiation exposure, changes in DNA methylation, and carcinogenesis are unclear. The aims of this study were (1) to examine whether low-level occupational radiation exposure induces genomic DNA hypomethylation; and (2) to determine the relationships between radiation exposure, genomic DNA hypomethylation and radiation-induced genomic instability (RIGI) in industrial radiographers. Genomic DNA methylation levels were measured in blood DNA from 40 radiographers and 28 controls using the LINE-1 pyrosequencing assay and the luminometric methylation assay. Further, the micronucleus-centromere assay was performed to measure aneuploidy of chromosomes 1 and 4 as a marker of delayed RIGI. Genomic DNA methylation levels were significantly lower in radiographers than those in controls. LINE-1 hypomethylation was not significantly correlated with recent 1-year, recent 3-year, or total cumulative radiation doses in radiographers; however, LINE-1 hypomethylation significantly correlated with the cumulative radiation dose without recent 3-year exposure data (D3dose, r = -0.39, P < 0.05). In addition, LINE-1 hypomethylation was a significant contributor to aneuploidy frequency by D3dose (F (2, 34) = 13.85, P < 0.001), in which a total of 45% of the variance in aneuploidy frequency was explained. Our results provide suggestive evidence regarding the delayed effects of low-dose occupational radiation exposure in radiographers and its association with LINE-1 hypomethylation; however, additional studies using more subjects are needed to fully understand the relationship between genomic DNA hypomethylation and RIGI. Environ. Mol. Mutagen. 60: 174-184, 2019. (c) 2018 Wiley Periodicals, Inc.
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