Metagenomic Mining and Functional Characterization of a Novel KG51 Bifunctional Cellulase/Hemicellulase from Black Goat Rumen
DC Field | Value | Language |
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dc.contributor.author | Lee, Kyung-Tai | - |
dc.contributor.author | Toushik, Sazzad Hossen | - |
dc.contributor.author | Baek, Jin-Young | - |
dc.contributor.author | Kim, Ji-Eun | - |
dc.contributor.author | Lee, Jin-Sung | - |
dc.contributor.author | Kim, Keun-Sung | - |
dc.date.available | 2019-03-07T04:35:03Z | - |
dc.date.issued | 2018-08 | - |
dc.identifier.issn | 0021-8561 | - |
dc.identifier.issn | 1520-5118 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/1872 | - |
dc.description.abstract | A novel KG51 gene was isolated from a metagenomic library of Korean black goat rumen and its recombinant protein was characterized as a bifunctional enzyme (cellulase/hemicellulase). In silico sequence and domain analyses revealed that the KG51 gene encodes a novel carbohydrate-active enzyme that possesses a salad-bowl-like shaped glycosyl hydrolase family 5 (GH5) catalytic domain but, at best, 41% sequence identity with other homologous GH5 proteins. Enzymatic profiles (optimum pH values and temperatures, as well as pH and thermal stabilities) of the recombinant KG51 bifunctional enzyme were also determined. On the basis of the substrate specificity data, the KG51 enzyme exhibited relatively strong cellulase (endo-beta-1,4-glucanase [EC 3.2.1.4]) and hemicellulase (mannan endo-beta-1,4-mannosidase [EC 3.2.1.78] and endo-beta-1,4-xylanase [EC 3.2.1.8]) activities, but no exo-beta-1,4-glucanase (EC 3.2.1.74), exo-beta-1,4-glucan cellobiohydrolase (EC 3.2.1.91), and exo-1,4-beta-xylosidase (EC 3.2.1.37) activities. Finally, the potential industrial applicability of the KG51 enzyme was tested in the preparation of prebiotic konjac glucomannan hydrolysates. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | Metagenomic Mining and Functional Characterization of a Novel KG51 Bifunctional Cellulase/Hemicellulase from Black Goat Rumen | - |
dc.type | Article | - |
dc.identifier.doi | 10.1021/acs.jafc.8b01449 | - |
dc.identifier.bibliographicCitation | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.66, no.34, pp 9034 - 9041 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000443653900014 | - |
dc.identifier.scopusid | 2-s2.0-85052323456 | - |
dc.citation.endPage | 9041 | - |
dc.citation.number | 34 | - |
dc.citation.startPage | 9034 | - |
dc.citation.title | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | - |
dc.citation.volume | 66 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | rumen | - |
dc.subject.keywordAuthor | cellulase | - |
dc.subject.keywordAuthor | hemicellulase | - |
dc.subject.keywordAuthor | bifunctional | - |
dc.subject.keywordAuthor | glycosyl hydrolase family 5 | - |
dc.subject.keywordPlus | BETA-MANNANASE | - |
dc.subject.keywordPlus | BUFFALO RUMEN | - |
dc.subject.keywordPlus | CELLULASE | - |
dc.subject.keywordPlus | LIBRARY | - |
dc.subject.keywordPlus | KONJAC | - |
dc.subject.keywordPlus | GLUCOMANNAN | - |
dc.subject.keywordPlus | ENZYMES | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | OLIGOSACCHARIDES | - |
dc.subject.keywordPlus | SACCHARIFICATION | - |
dc.relation.journalResearchArea | Agriculture | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Agriculture, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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