소리쟁이 뿌리 성분이 조골세포 분화에 미치는 영향

Abstract

Rumex crispus (Curled Dock, Polygonaceae) is a perennial wild plant used in traditional medicine as a laxative,astringent, and to treat blood and skin disease. The ethanol extract of R. crispus was obtained and its carbohydrate contentswere analyzed using high-performance anion-exchange chromatography with pulsed amperometric detection. The anaboliceffects of R. crispus in human osteoblastic MG-63 cells were investigated using the WST-8 assay, alkalinephosphatase (ALP)assay, and mineralization assay. The ethanol extract increased the proliferation of MG-63 cells and stimulated ALP activityin a dose-dependent manner over a 72-hrs period. Additionally, the ethanol extract dose-dependently stimulated the formationof bone nodules in MG-63 cells treated for 12 days. The ethyl acetate fraction from the ethanol extract did not affectosteoblast viability but induced an increase in ALP activity. In conclusion, the ethanol extract of R. crispus increases the proliferationand bone-forming activity of osteoblasts, and hence it could be used in the development of bone-forming stimulatorynutraceuticals and osteoporosis-related medicines.