Effect of PRX-1 Downregulation in the Type 1 Diabetes Microenvironment
- Authors
- Yoo, Jong-Sun; Lee, Yun-Jung; Hyung, Kyeong Eun; Yoon, Joo Won; Lee, Ik Hee; Park, So-Young; Hwang, Kwang Woo
- Issue Date
- Dec-2012
- Publisher
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Keywords
- Nitric oxide; Pancreatic beta-cell; Peroxiredoxin; Type 1 diabetes
- Citation
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, v.16, no.6, pp 463 - 468
- Pages
- 6
- Journal Title
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Volume
- 16
- Number
- 6
- Start Page
- 463
- End Page
- 468
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/19982
- DOI
- 10.4196/kjpp.2012.16.6.463
- ISSN
- 1226-4512
2093-3827
- Abstract
- Type 1 diabetes (T1D) is caused by dysregulation of the immune system in the pancreatic islets, which eventually leads to insulin-producing pancreatic beta-cell death and destabilization of glucose homeostasis. One of the major characteristics of T1D pathogenesis is the production of inflammatory mediators by macrophages that result in destruction or damage of pancreatic beta-cells. In this study the inflammatory microenvironment of T1D was simulated with RAW264.7 cells and MIN6 cells, acting as macrophages and pancreatic beta-cells respectably. In this setting, peroxiredoxin-1, an anti-oxidant enzyme was knocked down to observe its functions in the pathogenesis of T1D. RAW264.7 cells were primed with lipopolysaccharide and co-cultured with MIN6 cells while PRX-1 was knocked down in one or both cell types. Our results suggest that hindrance of PRX-1 activity or the deficiency of this enzyme in inflammatory conditions negatively affects pancreatic beta-cell survival. The observed decrease in viability of MIN6 cells seems to be caused by nitric oxide production. Additionally, it seems that PRX-1 affects previously reported protective activity of IL-6 in pancreatic beta cells as well. These results signify new, undiscovered roles for PRX-1 in inflammatory conditions and may contribute toward our understanding of autoimmunity.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.