Bacterial Lipopolysaccharides Induce Steroid Sulfatase Expression and Cell Migration through IL-6 Pathway in Human Prostate Cancer Cells
- Authors
- Im, Hee-Jung; Park, Na-Hee; Kwon, Yeo-Jung; Shin, Sangyun; Kim, Donghak; Chun, Young-Jin
- Issue Date
- 30-Nov-2012
- Publisher
- KOREAN SOC APPLIED PHARMACOLOGY
- Keywords
- Steroid sulfatase; Lipopolysaccharides; Interleukin-6; Tumor cell migration; PC-3
- Citation
- BIOMOLECULES & THERAPEUTICS, v.20, no.6, pp 556 - 561
- Pages
- 6
- Journal Title
- BIOMOLECULES & THERAPEUTICS
- Volume
- 20
- Number
- 6
- Start Page
- 556
- End Page
- 561
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/20012
- DOI
- 10.4062/biomolther.2012.20.6.556
- ISSN
- 1976-9148
2005-4483
- Abstract
- Steroid sulfatase (STS) is responsible for the conversion of estrone sulfate to estrone that can stimulate growth in endocrine-dependent tumors such as prostate cancer. Although STS is considered as a therapeutic target for the estrogen-dependent diseases, cellular function of STS are still not clear. Previously, we found that tumor necrosis factor (TNF)-alpha significantly enhances steroid sulfatase expression in PC-3 human prostate cancer cells through PI3K/Akt-dependent pathways. Here, we studied whether bacterial lipopolysaccharides (LPS) which are known to induce TNF-alpha may increase STS expression. Treatment with LPS in PC-3 cells induced STS mRNA and protein in concentration- and time-dependent manners. Using luciferase reporter assay, we found that LPS enhanced STS promoter activity. Moreover, STS expression induced by LPS increased PC-3 tumor cell migration determined by wound healing assay. We investigated that LPS induced IL-6 expression and IL-6 increased STS expression. Taken together, these data strongly suggest that LPS induces STS expression through IL-6 pathway in human prostate cancer cells.
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