Development of Estimation Methods of Skin Oxidation and Evaluation of Anti-Oxidative Effects of Genistein in Topical Formulations
- Authors
- Kim, Seong Yeon; Na, Yeon Joo; Kim, Dongju; Kim, Yeongseok; Kim, Hyeong Min; Hwang, Sung-Ha; Kwak, Jiyeon; Kuh, Hyo-Jeong; Lee, Jaehwi
- Issue Date
- Jun-2012
- Publisher
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Keywords
- Oxidation; Skin; Hydrogen peroxide; Genistein
- Citation
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, v.16, no.3, pp 205 - 209
- Pages
- 5
- Journal Title
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Volume
- 16
- Number
- 3
- Start Page
- 205
- End Page
- 209
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/20302
- DOI
- 10.4196/kjpp.2012.16.3.205
- ISSN
- 1226-4512
2093-3827
- Abstract
- The objective of the present study was to establish the method of measurement of hydrogen peroxide and to estimate the anti-oxidative effect of genistein in the skin. UVB induced skin oxidation and anti-oxidative effect of genistein formulations were evaluated by determining levels of hydrogen peroxide. The mechanism involved in the determination of hydrogen peroxide is based on a color reaction between ferric ion (Fe3+) and xylenol orange, often called FOX assay and subsequent monitoring of absorbance values of the reactant at 540 nm. The reaction was to some extent pH-dependent and detection sensitivity was greatest at pH 1.75. Genistein liposomal gel demonstrated better anti-oxidative effect with regard to lowering hydrogen peroxide levels elevated by UVB irradiation compared to genistein-suspended gel. A linear relationship has been observed between anti-oxidative effect of genistein and drug deposition in the skin tissue. Genistein liposomal gel resulting in the localization of the drug in the deeper skin led to improved anti-oxidative effect compared to genistein gel. The suggested method for evaluation of oxidation of the skin can be used as a tool to screen effective anti-oxidative agents and their delivery systems acting on the skin.
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