Dynein regulates cell migration depending on substrate rigidityopen access
- Authors
- Kim, Daehwan; You, Eunae; Rhee, Sangmyung
- Issue Date
- Mar-2012
- Publisher
- Demetrios A. Spandidos Ed. & Pub.
- Keywords
- cell migration; tractional force; dynein; myosin; microtubule; 3D matrix
- Citation
- International Journal of Molecular Medicine, v.29, no.3, pp 440 - 446
- Pages
- 7
- Journal Title
- International Journal of Molecular Medicine
- Volume
- 29
- Number
- 3
- Start Page
- 440
- End Page
- 446
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/20454
- DOI
- 10.3892/ijmm.2011.867
- ISSN
- 1107-3756
1791-244X
- Abstract
- The mechanical environment in combination with biochemical signaling is an important regulatory factor for cellular physiology including tissue development, cell motility and differentiation. Exerting a tractional force triggered by myosin-dependent cell contractility is known to be an indispensible element of cell migration in a mechanically stiff environment such as a 2D planar surface. However, a number of reports have argued that the requirement of myosin activity for cell migration is limited by cell type and the environment. In this study, we present evidence that dynein, a minus end-directed microtubule motor, plays a central role in cell migration in the absence of tractional force. Interfering with the dynein activity through a dynein-specific inhibitor, erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA), dramatically inhibited 2D migration of the fibroblast when cell contractility was blocked by Rho kinase or a myosin inhibitor, although EHNA itself did not affect cell migration. Cell migration in 3D soft collagen matrices, where the cell exerts a relatively low tractional force compared to that on a 2D stiff surface, is also profoundly inhibited by dynein intermediate chain (DIC) silencing regardless of the presence of myosin activity. In addition, DIC-silenced cells on a soft acrylamide surface show decreased migration without blockade of myosin activity. Taken together, our results suggest that dynein may be a primary regulatory factor for cell migration when a cell is in a mechanically low-tension environment, such as in a 3D matrix.
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