Interspecies Interaction of Signal Peptide PapR Secreted by Bacillus cereus and Its Effect on Production of Antimicrobial Peptide
- Authors
- Yeo, In-Cheol; Lee, Nam Keun; Cha, Chang-Jun; Hahm, Young Tae
- Issue Date
- Feb-2012
- Publisher
- HUMANA PRESS INC
- Keywords
- Bacillus cereus group; PapR; Bacillus subtilis; Antimicrobial peptide; Interspecies interaction
- Citation
- APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.166, no.3, pp 700 - 710
- Pages
- 11
- Journal Title
- APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
- Volume
- 166
- Number
- 3
- Start Page
- 700
- End Page
- 710
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/20555
- DOI
- 10.1007/s12010-011-9459-9
- ISSN
- 0273-2289
1559-0291
- Abstract
- This study was carried out to investigate the interspecies interaction of PapR peptide secreted by Bacillus cereus on production of BSAP-254, an antimicrobial peptide produced by Bacillus subtilis SC-8 isolated from the Korean fermented soybean paste and exhibited narrow antagonistic activity against the B. cereus group, but not against other foodborne pathogens. PapR is a signal peptide that activates PlcR, which is a pleiotropic regulator controlling the expression of various virulence factors in B. cereus. When B. subtilis SC-8 was co-cultured with B. cereus, it completely inhibited the growth of B. cereus within 12 h, and the rate of BSAP-254 production was increased 34.2% at 12 h. Furthermore, 5 mu M of synthetic PapR peptide added to the culture of B. subtilis SC-8 increased the rate of BSAP-254 production up to 59.7%. The growth of B. subtilis SC-8, however, was not significantly different with or without the addition of PapR. When B. cereus papR mutant was co-cultured with B. subtilis SC-8, the growth of the mutant was not inhibited and the rate of BSAP-254 production was decreased by 45%.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/20555)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.