GENE EXPRESSION PROFILING OF A COLD-SHOCKED EARTHWORM EISENIA andrei
- Authors
- Kim, Hyeng-Soo; Ahn, Chi Hyun; Park, Yong-Soo; Park, Hum Dai; Koh, Ki Seok; Ryool, Zae Young; Park, Soon Cheol; Lee, Sanggyu
- Issue Date
- Jan-2012
- Publisher
- CRYO LETTERS
- Keywords
- earthworm; Eisenia andrei; cold stress; SAGE; transcription; gene expression
- Citation
- CRYOLETTERS, v.33, no.1, pp 1 - 11
- Pages
- 11
- Journal Title
- CRYOLETTERS
- Volume
- 33
- Number
- 1
- Start Page
- 1
- End Page
- 11
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/20632
- ISSN
- 0143-2044
- Abstract
- To identify genes that are modulated under cold-stress conditions in the earthworm Eisenia andrei, we performed a genome-wide analysis of gene expression in cold-shocked earthworms by using Serial Analysis of Gene Expression (SAGE). We identified 5,977 and 5,407 unique SAGE tags under normal and cold-stressed conditions, respectively. The majority of the SAGE tags did not match to any known expressed sequences, due to a paucity of expression data in earthworms. We converted the statistically significant SAGE tags for the cold-stressed condition into expressed sequence tags (ESTs), and the results showed that particular genes associated with energy homeostasis, cellular defense mechanisms, and ion balance were up-regulated or down-regulated. We constructed a regulatory network of some of these genes and identified rps-6 as a core gene in the cold-response regulatory-gene network. Our data provide a baseline for gene expression studies of cold shock in the Lumbricidae.
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