Determination of three preservatives, cresol, chlorocresol and benzethonium, in drugs by high performance liquid chromatography-ultraviolet (HPLC-UV) detectionDetermination of three preservatives, cresol, chlorocresol and benzethonium, in drugs by high performance liquid chromatography–ultraviolet (HPLC–UV) detection
- Authors
- Lee, J.; Shin, S.; Shin, H.; Huh, Y.; Lee, S.; Kim, D.-H.; Lee, S.; Kim, Y.-O.; Han, S.B.; Lee, J.; Park, J.H.; Kwon, S.W.
- Issue Date
- Feb-2012
- Publisher
- 한국약제학회
- Keywords
- Benzethonium; Chlorocresol; Cresol; HPLC-UV; Preservatives
- Citation
- Journal of Pharmaceutical Investigation, v.42, no.1, pp 47 - 50
- Pages
- 4
- Journal Title
- Journal of Pharmaceutical Investigation
- Volume
- 42
- Number
- 1
- Start Page
- 47
- End Page
- 50
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/20861
- DOI
- 10.1007/s40005-012-0008-5
- ISSN
- 2093-5552
2093-6214
- Abstract
- High performance liquid chromatography-ultraviolet (HPLC-UV) detection method was performed to simultaneously determine three preservatives: cresol, chlorocresol and benzethonium. According to the current Korean Pharmacopeia Codex, the only-available analytical method for discerning the three preservatives uses thin-layer chromatography, which does not provide accurate detection or quantification. Therefore, this study focused on developing a new HPLC-UV method which gives fast, accurate and sensitive outcomes. The experiment was done using Phenomenex Gemini column [C18 (250 × 4.6 mm, 5 μm)] and the mobile system was methanol and 0.01 M potassium phosphate (monobasic) in water (pH = 3.3). The developed method was validated through linearity, range, system suitability, precision, accuracy, recovery and inter-laboratory variation. © 2012 The Korean Society of Pharmaceutical Sciences and Technology and Springer Dordrecht.
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Collections - College of Pharmacy > School of Pharmacy > 1. Journal Articles
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