Proteomic Analysis on Acetate Metabolism in Citrobacter sp BL-4
- Authors
- Kim, Young-Man; Lee, Sung-Eun; Park, Byeoung-Soo; Son, Mi-Kyung; Jung, Young-Mi; Yang, Seung-Ok; Choi, Hyung-Kyoon; Hur, Sung-Ho; Yum, Jong Hwa
- Issue Date
- 2011
- Publisher
- IVYSPRING INT PUBL
- Keywords
- Citrobacter sp BL-4; proteomics; H-1-NMR; acetate metabolism; polyglucosamine production
- Citation
- INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, v.8, no.1, pp 66 - 78
- Pages
- 13
- Journal Title
- INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
- Volume
- 8
- Number
- 1
- Start Page
- 66
- End Page
- 78
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/20945
- DOI
- 10.7150/ijbs.8.66
- ISSN
- 1449-2288
- Abstract
- Mass production of glucosamine (GlcN) using microbial cells is a worthy approach to increase added values and keep safety problems in GlcN production process. Prior to set up a microbial cellular platform, this study was to assess acetate metabolism in Citrobacter sp. BL-4 (BL-4) which has produced a polyglucosamine PGB-2. The LC-MS analysis was conducted after protein separation on the 1D-PAGE to accomplish the purpose of this study. 280 proteins were totally identified and 188 proteins were separated as acetate-related proteins in BL-4. Acetate was converted to acetyl-CoA by acetyl-CoA synthetase up-regulated in the acetate medium. The glyoxylate bypass in the acetate medium was up-regulated with over-expression of isocitrate lyases and 2D-PAGE confirmed this differential expression. Using H-1-NMR analysis, the product of isocitrate lyases, succinate, increased about 15 times in the acetate medium. During acetate metabolism proteins involved in the lipid metabolism and hexosamine biosynthesis were over-expressed in the acetate medium, while proteins involved in TCA cycle, pentose phosphate cycle and purine metabolism were down-regulated. Taken together, the results from the proteomic analysis can be applied to improve GlcN production and to develop metabolic engineering in BL-4.
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