Bacillus manliponensis sp nov., a new member of the Bacillus cereus group isolated from foreshore tidal flat sediment
- Authors
- Jung, Min Young; Kim, Joong-Su; Paek, Woon Kee; Lim, Jeongheui; Lee, Hansoo; Kim, Pyoung Il; Ma, Jin Yeul; Kim, Wonyong; Chang, Young-Hyo
- Issue Date
- Dec-2011
- Publisher
- MICROBIOLOGICAL SOCIETY KOREA
- Keywords
- Bacillus cereus group; Bacillus manliponensis sp nov.; phylogenetic; new species
- Citation
- JOURNAL OF MICROBIOLOGY, v.49, no.6, pp 1027 - 1032
- Pages
- 6
- Journal Title
- JOURNAL OF MICROBIOLOGY
- Volume
- 49
- Number
- 6
- Start Page
- 1027
- End Page
- 1032
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21076
- DOI
- 10.1007/s12275-011-1049-6
- ISSN
- 1225-8873
1976-3794
- Abstract
- A Gram-positive, endospore-forming, new Bacillus species, strain BL4-6(T), was isolated from tidal flat sediment of the Yellow Sea. Strain BL4-6(T) is a straight rod, with motility by peritrichate flagella. The cell wall contains meso-diaminopimelic acid, and the major respiratory quinone is menaquinone-7. The major fatty acids are iso-C-15:0 and summed feature 3 (containing C-16:1 omega 7c/iso-C-15:0 2OH, and/or iso-C-15:0 2OH/C-16:1 omega 7c). Cells are catalase-positive and oxidase-negative. The G+C content of the genomic DNA is 38.0 mol%. Based on a comparative 16S rRNA gene sequence analysis, the isolate belongs to the genus Bacillus, forms a clade with the Bacillus cereus group, and is closely related to Bacillus mycoides (98.5%), Bacillus cereus (98.5%), Bacillus anthracis (98.4%), Bacillus thuringiensis (98.4%), Bacillus weihenstephanensis (98.1%), and Bacillus pseudomycoides (97.5%). The isolate showed less than 85% similarity of the gyrA gene sequence and below 95% similarity of the rpoB gene sequence to the members of this group. DNA-DNA relatedness between strain BL4-6(T) and B. cereus group was found to be in a range of 22.8-42.3%, and thus BL4-6(T) represents a unique species. On the basis of these studies, strain BL4-6(T) (=KCTC 13319(T) =JCM 15802(T)) is proposed to represent the type strain of a novel species, Bacillus manliponensis sp. nov.
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