A plant-specific tau class glutathione S-transferase from Oryza sativa with very high activity against 1-chloro-2,4-dinitrobenzene and chloroacetanilide herbicides
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jo, Hyun-Joo | - |
dc.contributor.author | Lee, Jin-Ju | - |
dc.contributor.author | Kong, Kwang-Hoon | - |
dc.date.available | 2019-05-29T11:32:23Z | - |
dc.date.issued | 2011-11 | - |
dc.identifier.issn | 0048-3575 | - |
dc.identifier.issn | 1095-9939 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21150 | - |
dc.description.abstract | A plant-specific tau class GST gene homolog was successfully cloned from an Oryza sativa cDNA library by PCR using oligonucleotide primers based on the OsGSTU4 (GenBank Accession No. AF309378) sequence. The cDNA was composed of a 720-bp open reading frame encoding 239 amino acids. The deduced amino acid sequence of this gene shared over 65% sequence identity with the sequences of the tau class TaGST28e45 and ZmGST42. Conversely, the OsGSTU4 sequence showed very low identity to the GST sequences of phi, theta and zeta classes. This gene was expressed in Escherichia coli with the pET vector system, and the gene product was purified to homogeneity using GSH-Sepharose affinity column chromatography. The expressed OsGSTU4 formed a homodimer with subunits of approximately 25.5 kDa. OsGSTU4 displayed very high activity toward 1-chloro-2,4-dinitrobenzene. The activity of the OsGSTU4 was significantly inhibited by S-hexylglutathione and hematin. Plant OsGSTU4 had a unique herbicide specificity and played an important role in the detoxification reaction against fluorodifen and chloroacetanilide herbicides. (C) 2011 Elsevier Inc. All rights reserved. | - |
dc.format.extent | 5 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | A plant-specific tau class glutathione S-transferase from Oryza sativa with very high activity against 1-chloro-2,4-dinitrobenzene and chloroacetanilide herbicides | - |
dc.type | Article | - |
dc.identifier.doi | 10.1016/j.pestbp.2011.10.005 | - |
dc.identifier.bibliographicCitation | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, v.101, no.3, pp 265 - 269 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000297879600018 | - |
dc.identifier.scopusid | 2-s2.0-81555219348 | - |
dc.citation.endPage | 269 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 265 | - |
dc.citation.title | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY | - |
dc.citation.volume | 101 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordAuthor | Glutathione S-transferase | - |
dc.subject.keywordAuthor | Herbicide activity | - |
dc.subject.keywordAuthor | Oryza sativa GST | - |
dc.subject.keywordAuthor | Rice | - |
dc.subject.keywordAuthor | Tau class GST | - |
dc.subject.keywordPlus | GENE FAMILY | - |
dc.subject.keywordPlus | MOLECULAR-CLONING | - |
dc.subject.keywordPlus | DRAFT SEQUENCE | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | MAIZE | - |
dc.subject.keywordPlus | DETOXIFICATION | - |
dc.subject.keywordPlus | EVOLUTION | - |
dc.subject.keywordPlus | ROLES | - |
dc.subject.keywordPlus | GST | - |
dc.subject.keywordPlus | L. | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Entomology | - |
dc.relation.journalResearchArea | Physiology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Entomology | - |
dc.relation.journalWebOfScienceCategory | Physiology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
84, Heukseok-ro, Dongjak-gu, Seoul, Republic of Korea (06974)02-820-6194
COPYRIGHT 2019 Chung-Ang University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.