Interleukin-34 Regulates Th1 and Th17 Cytokine Production by Activating Multiple Signaling Pathways through CSF-1R in Chicken Cell Linesopen access
- Authors
- Truong, Anh Duc; Hong, Yeojin; Lee, Janggeun; Lee, Kyungbaek; Kil, Dong Yong; Lillehoj, Hyun S.; Hong, Yeong Ho
- Issue Date
- Jun-2018
- Publisher
- MDPI
- Keywords
- cytokines; chicken; interleukin-34; CSF-1R; signaling pathway
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.19, no.6
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- Volume
- 19
- Number
- 6
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/2143
- DOI
- 10.3390/ijms19061665
- ISSN
- 1422-0067
1422-0067
- Abstract
- Interleukin-34 (IL-34) is a newly recognized cytokine with functions similar to macrophage colony-stimulating factor 1. It is expressed in macrophages and fibroblasts, where it induces cytokine production; however, the mechanism of chicken IL-34 (chIL-34) signaling has not been identified to date. The aim of this study was to analyze the signal transduction pathways and specific biological functions associated with chIL-34 in chicken macrophage (HD11) and fibroblast (OU2) cell lines. We found that IL-34 is a functional ligand for the colony-stimulating factor receptor (CSF-1R) in chicken cell lines. Treatment with chIL-34 increased the expression of Th1 and Th17 cytokines through phosphorylation of tyrosine and serine residues in Janus kinase (JAK) 2, tyrosine kinase 2 (TYK2), signal transducer and activator of transcription (STAT) 1, STAT3, and Src homology 2-containing tyrosine phosphatase 2 (SHP-2), which also led to phosphorylation of NF-B1, p-mitogen-activated protein kinase kinase kinase 7 (TAK1), MyD88, suppressor of cytokine signaling 1 (SOCS1), and extracellular signal-regulated kinase 1 and 2 (ERK1/2). Taken together, these results suggest that chIL-34 functions by binding to CSF-1R and activating the JAK/STAT, nuclear factor B (NF-B), and mitogen-activated protein kinase signaling pathways; these signaling events regulate cytokine expression and suggest roles for chIL-34 in innate and adaptive immunity.
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