The Relaxing Effect of alpha-Defensin 1 on the Adrenergic Responses of Rat Bladder
- Authors
- Lee, Shin Young; Kim, Don Kyu; Kim, Kyung Do; Myung, Soon Chul; Lee, Moo Yeol
- Issue Date
- Jun-2011
- Publisher
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Keywords
- alpha-Defensin; Detrusor smooth muscle; NF-kappa B; Adrenergic system
- Citation
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, v.15, no.3, pp 143 - 147
- Pages
- 5
- Journal Title
- KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY
- Volume
- 15
- Number
- 3
- Start Page
- 143
- End Page
- 147
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21514
- DOI
- 10.4196/kjpp.2011.15.3.143
- ISSN
- 1226-4512
2093-3827
- Abstract
- Defensins, cysteine-rich cationic polypeptides released from neutrophils, are known to have powerful antimicrobial properties. In this study, we sacrificed 30 rats to investigate the effects of alpha-defensin 1 on detrusor muscle contractions in isolated rat bladder. From the experiments we found relaxing effects of alpha-defensin 1 on the contractions induced by phenylephrine (PE) but not by bethanechol (BCh) in the detrusor smooth muscles. To determine the mechanisms of the effects of alpha-defensin 1, the changes of effects on PE-induced contraction by alpha-defensin 1 pretreatment were observed after pretreatment of Rho kinase inhibitor (Y-27632), protein kinase C (PKC) inhibitor (Calphostin C), potent activator of PKC (PDBu; phorbol 12,13-dibutyrate), and NF-kappa B inhibitors (PDTC; pyrrolidinedithiocarbamate and sulfasalazine). The contractile responses of PE (10(-9)similar to 10(-4) M) were significantly decreased in some concentrations of alpha-defensin 1 (5x10(-9) and 5x10(-9) M). When strips were pretreated with NF-kappa B inhibitors (PDTC and sulfasalazine; 10(-7)similar to 10(-6) M), the relaxing responses by alpha-defensin 1 pretreatment were disappeared. The present study demonstrated that alpha-defensin 1 has relaxing effects on the contractions of rat detrusor muscles, through NF-kappa B pathway. Further studies in vivo are required to clarify whether alpha-defensin 1 might be clinically related with bladder dysfunction by inflammation process.
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