Chromosome-Encoded AmpC and CTX-M Extended-Spectrum beta-Lactamases in Clinical Isolates of Proteus mirabilis from Korea
- Authors
- Song, Wonkeun; Kim, Juwon; Bae, Il Kwon; Jeong, Seok Hoon; Seo, Young Hee; Shin, Jong Hee; Jang, Sook Jin; Uh, Young; Shin, Jeong Hwan; Lee, Mi-Kyung; Lee, Kyungwon
- Issue Date
- Apr-2011
- Publisher
- AMER SOC MICROBIOLOGY
- Citation
- ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, v.55, no.4, pp 1414 - 1419
- Pages
- 6
- Journal Title
- ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
- Volume
- 55
- Number
- 4
- Start Page
- 1414
- End Page
- 1419
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21627
- DOI
- 10.1128/AAC.01835-09
- ISSN
- 0066-4804
1098-6596
- Abstract
- Among 222 Proteus mirabilis clinical isolates collected from 17 hospitals in Korea in 2008, 28 (12.6%) and 8 (3.6%) isolates exhibited extended-spectrum beta-lactamase (ESBL) and AmpC phenotypes, respectively. The most common type of ESBL gene identified by PCR and sequencing experiments was bla(CTX-M-14a) (n = 12). The bla(CTX-M-90) (n = 4), bla(CTX-M-15) (n = 3), bla(CTX-M-12) (n = 3), bla(CTX-M-2) (n = 2), bla(CTX-M-14b) (n = 1), bla(TEM-52) (n = 5), and bla(SHV-12) (n = 1) genes were also detected. Eight isolates carried an AmpC beta-lactamase gene, such as bla(CMY-2) (n = 6) or bla(DHA-1) (n = 2). All bla genes encoding CTX-M-1- and CTX-M-9-type enzymes and all bla(CMY-2) genes were preceded by ISEcp1-like elements. The bla(CTX-M-2) gene found in two isolates was located on a complex class 1 integron. The bla(DHA-1) gene was preceded by a transcriptional regulator gene and was followed by phage shock protein genes. The bla(CTX-M) genes were located on the chromosome in 21 isolates. A plasmid location for the bla(CTX-M) gene was found in only four isolates: the bla(CTX-M-14a) gene was located on similar to 150-kbp IncA/C plasmids in three isolates and on a similar to 50-kbp IncN plasmid in one isolate. The bla(TEM-52) gene was located on similar to 50-kbp IncN plasmids in all five isolates. The AmpC beta-lactamase genes were located on the chromosome in seven of eight isolates; one isolate carried the bla(CMY-2) gene on a similar to 150-kbp IncA/C plasmid. Our results show that a chromosomal location of CTX-M ESBL and AmpC beta-lactamase genes in P. mirabilis is no longer an unusual phenomenon in hospital environments.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > College of Medicine > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21627)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.