A functional promoter polymorphism-607G > C of WNT10B is associated with abdominal fat in Korean female subjects
- Authors
- Kim, Il Chul; Cha, Min Ho; Kim, Dong Min; Lee, Haeyong; Moon, Jin Seok; Choi, Sun Mi; Kim, Kil Soo; Yoon, Yoosik
- Issue Date
- Mar-2011
- Publisher
- ELSEVIER SCIENCE INC
- Keywords
- WNT10B; Polymorphism; SNP; Obesity; Fat; Association
- Citation
- JOURNAL OF NUTRITIONAL BIOCHEMISTRY, v.22, no.3, pp 252 - 258
- Pages
- 7
- Journal Title
- JOURNAL OF NUTRITIONAL BIOCHEMISTRY
- Volume
- 22
- Number
- 3
- Start Page
- 252
- End Page
- 258
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21683
- DOI
- 10.1016/j.jnutbio.2010.02.002
- ISSN
- 0955-2863
1873-4847
- Abstract
- WNT10B has been implicated as a potential regulator of adipogenesis in cellular and animal models of obesity. In this study, we attempted to characterize the associations between common genetic polymorphisms of WNT10B and fat accumulation in a sample of 1029 Korean female subjects. Direct sequencing of genomic DNAs of 45 subjects identified six common single-nucleotide polymorphisms (SNPs) of WNT10B, which were in almost complete linkage disequilibrium. Among the six SNPs, -607G>C (rs833840) showed differential nuclear factor binding in an electrophoretic mobility shift assay and differential promoter activity in a reporter assay, implicating it as a functional regulatory SNP. When body compositions of the subjects determined using bio-impedance analysis were compared according to their -607G>C genotype, only body fat mass showed a significant association. Body masses of protein, mineral and water showed no association. For more accurate evaluation of the effects of -607G>C genotype on body fat, cross-sectional fat areas of the subjects measured by abdominal computed tomography were compared. Genotype of -607G>C was significantly associated with abdominal total fat and abdominal subcutaneous fat areas (P=.009 and P=.007 in recessive model, respectively). Of the 1029 subjects, 576 were treated with a 1 month very low calorie diet and changes of body weight and composition were compared with -607G>C genotype. No significant associations were evident. This study is the first report of the association of common genetic polymorphism of WNT10B with human fat accumulation. (C) 2011 Elsevier Inc. All rights reserved.
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