Studies on a Vibrio vulnificus Functional Ortholog of Escherichia coli RNase E Imply a Conserved Function of RNase E-like Enzymes in Bacteria
DC Field | Value | Language |
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dc.contributor.author | Lee ,Minho | - |
dc.contributor.author | Yeom ,Ji-Hyun | - |
dc.contributor.author | Jeon, Che Ok | - |
dc.contributor.author | Lee, Kangseok | - |
dc.date.available | 2019-05-29T13:39:17Z | - |
dc.date.issued | 2011-03 | - |
dc.identifier.issn | 0343-8651 | - |
dc.identifier.issn | 1432-0991 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/21700 | - |
dc.description.abstract | RNase E (Rne) plays a key role in the processing and degradation of RNA in Escherichia coli. In the genome of Vibrio vulnificus, one open reading frame potentially encodes a protein homologous to E. coli RNase E, designated RNase EV, which N-terminal (1-500 amino acids) has 86.4% amino acid identity to the N-terminal catalytic part of RNase E (N-Rne). Here, we report that both the full-length and the N-terminal part of RNase EV (N-RneV) functionally complement E. coli RNase E and their expression consequently supports normal growth of RNase E-depleted E. coli cells. E. coli cells expressing N-RneV showed copy numbers of ColE1-type plasmid similar to that of E. coli cells expressing N-Rne, indicating in vivo ribonucleolytic activity of N-RneV on RNA I, an antisense regulator of ColE1-type plasmid replication. In vitro cleavage assays further showed that N-RneV has cleavage activity and specificity of RNase E on RNase E-targeted sequence of RNA I (BR13). Our findings suggest that RNase E-like proteins have conserved enzymatic properties that determine substrate specificity across species. | - |
dc.format.extent | 5 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | SPRINGER | - |
dc.title | Studies on a Vibrio vulnificus Functional Ortholog of Escherichia coli RNase E Imply a Conserved Function of RNase E-like Enzymes in Bacteria | - |
dc.type | Article | - |
dc.identifier.doi | 10.1007/s00284-010-9771-6 | - |
dc.identifier.bibliographicCitation | CURRENT MICROBIOLOGY, v.62, no.3, pp 861 - 865 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.wosid | 000287754500026 | - |
dc.identifier.scopusid | 2-s2.0-79954439014 | - |
dc.citation.endPage | 865 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 861 | - |
dc.citation.title | CURRENT MICROBIOLOGY | - |
dc.citation.volume | 62 | - |
dc.type.docType | Article | - |
dc.publisher.location | 미국 | - |
dc.subject.keywordPlus | DEGRADOSOME | - |
dc.subject.keywordPlus | RRAA | - |
dc.subject.keywordPlus | ABUNDANCE | - |
dc.subject.keywordPlus | MUTATION | - |
dc.subject.keywordPlus | CLONING | - |
dc.subject.keywordPlus | DOMAIN | - |
dc.subject.keywordPlus | GENE | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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