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Stereoisomers of Castasterone, 3-Epicastasterone and 2,3-Diepicastasterone, in Immature Seeds of Phaseolus vulgaris
- Authors
- Lee, Sang Cheul; Joo, Se-Hwan; Kim, Seong-Ki
- Issue Date
- Feb-2011
- Publisher
- SPRINGER HEIDELBERG
- Keywords
- Brassinosteroids; 3-Epicastasterone; 2,3-Diepicastasterone; Structure elucidation; Biological activity
- Citation
- JOURNAL OF PLANT BIOLOGY, v.54, no.1, pp 10 - 14
- Pages
- 5
- Journal Title
- JOURNAL OF PLANT BIOLOGY
- Volume
- 54
- Number
- 1
- Start Page
- 10
- End Page
- 14
- ISSN
- 1226-9239
1867-0725
- Abstract
- A capillary GC-MS analysis revealed that two stereoisomers of castasterone are contained in immature seeds of Phaseolus vulgaris. 400 MHz proton NMR analysis of the stereoisomers determined they are A ring epimers of castasterone, 3-epicastasterone and 2,3-diepicastasterone. In rice lamina inclination assay, 3-epicastasterone and 2,3-diepicastasterone showed reduced biological activity than that of castasterone. Together with our previous finding that 2-epicastasterone exhibits a less biological activity than CS, this result indicates that epimerization of hydroxyl at C-2 or/and C-3 is/are inactive processes to reduce biological activity of CS after exerting as a bioactive brassinosteroid in P. vulgaris.
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